Project description:E. coli cultures were exposed to green or red CdTe Quantum Dots 50 µg/ml during 15 min. Total RNA was extracted and cDNA labeled probes were generated by reverse transcription using Alexa 555 and Alexa 647 fluorophores. These probes were used to hybridize genomic slides containing genomic arrays to determine global transcriptional changes.
Project description:Investigation of whole transcriptional changes in F. verticillioides FRC M-3125 when exposed to 5 μg/ml pyrrocidine A (PA), 20 μg/ml pyrrocidine B (PB), 50 μg/ml 2-benzoxazolinone (BOA), 50 μg/ml 2-oxindole (OXD), 50 μg/ml 2-coumaranone (CMN), or 50 μg/ml chlorzoxazone (CZX). Cultures were harvested one hour after exposure. Assessed in reference to control cultures of M-3125 exposed to DMSO (0.5% final concentration) since all the above compounds were dissolved in DMSO.
Project description:We reported changes in RNA methylation levels in A549 cells caused by black phosphorus quantum dots and titanium dioxide nanoparticles.
Project description:Human RPE-19 cells were treated with either Trolox (50 µg/mL), lutein (10 µg/mL) or gac peel extracts (0-200 µg/mL) for 12, 24, 48 or 72 h before being exposed to 500 µM H2O2 for 24 h prior to RNA collection. Results were presented as the fold change compared to untreated controls ± SEM (n=3), different letters indicated statistical significance at p≤0.05 between different treatments (Analysis by One-way ANOVA with Turkey’s LSD test).
Project description:To identify differentially expressed genes and key biological pathways that define toxicity following nanoparticles exposure, we performed microarray analyses on PMA-differentiated THP-1 macrophages exposed for 4 h to 8 µg/mL of NP ZnO (50 % cell viability) and to 2 µg/mL of NP ZnO (50 % cell viability divided by 4 ). This project has received funding from the European Union’s Horizon 2020 research and innovation programme under grant agreement No. 686098
Project description:In this study we investigate the transcriptomic response of Escherichia coli to CdTe-2.4 and benign CdSe-2.4 quantum dots, each with and without illumination to elucidate the phototherapeutic effect of CdTe-2.4. Our analysis sought to separate the transcriptomic responses of E. coli to the presence of superoxide and the presence of cadmium chalcogenide nanoparticles. We found eight genes to be consistently differentially expressed as a response to superoxide generation, and these genes demonstrate a consistent association with the DNA damage response and deactivation of iron-sulfur clusters, characteristic of a superoxide response. We found eighteen genes associated the presence of cadmium-based quantum dots, in isolation from the superoxide effect. In further analysis of these genes, we performed both amino acid supplementation and gene knockout experiments, identifying the importance of leucyl-tRNA downregulation as a cadmium-based QD response, as well as reinforcing the relationship between CdTe-2.4 stress and iron-sulfur clusters through the gene tusA. This study demonstrates the transcriptomic response of E. coli to CdTe-2.4 and CdSe-2.4 quantum dots and parses the different effects of superoxide versus material effects on the bacteria. Our findings may provide useful information towards the development of quantum dot-based antibacterial therapy in the future.
Project description:The objective was to investigate the toxic effect of hydroxyl-modified Graphene quantum dots (GQD) on normal human esophageal epithelial cells at gene expression level. Gene expression profilings between GQD and vehicle treated cells were compared.
Project description:Investigation of whole genome gene expression level changes in F. verticillioides FRC M-3125 when exposed to 50 ug/ml 2-benzoxazolinone (BOA). Cultures were harvested two hours after exposure. Assessed in reference to control cultures of M-3125 exposed to ethanol (1% final concentration) since BOA was dissolved in ethanol.
