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Mus musculus

ABSTRACT: RNA-SEQ analysis of single cells extracted from the metanephric mesenchyme of E11.5 Crym-EGFP transgenic mice

PROVIDER: PRJNA254485 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR1508620.fastq.gz	Fastqsanger.gz
	SRR1508630.fastq.gz	Fastqsanger.gz
	SRR1508640.fastq.gz	Fastqsanger.gz
	SRR1508650.fastq.gz	Fastqsanger.gz
	SRR1508660.fastq.gz	Fastqsanger.gz

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RNA-SEQ analysis of single cells extracted from the metanephric mesenchyme of Embryonic day 11.5 (E11.5) Crym-EGFP transgenic mice.

Project description:We used micro-dissection with FACS sorting techniques to isolate single cells from the metanephric mesenchyme of the E11.5 developing kidney. A subset of these single cell populations is analysed individually via Fluidigm single cell analysis. This analysis will determine the transcriptional profile of each cell type, identify compartment specific transcripts, compartment specific transcript isoforms and cell-type specific long-noncoding RNAs. In addition the unbiased nature of RNA-SEQ will potentially identify novel transcripts that have not been annotated in the database. Kidneys are harvested from Tg(Crym-EGFP)GF82Gsat mice. Single cells are extracted from E11.5 metanephric mesenchyme using manual micro-dissection techniques. A subset of these cells is analyzed individually via Fluidigm single cell analysis. The long term goal is to generate a transcriptional atlas of the developing kidney.

2014-08-01 | E-GEOD-59129 | biostudies-arrayexpress

Complete embryonic E11.5 metanephric kidney analysis of wild type and Hoxa11, Hoxd11 null targeted mice

2006-05-01 | GSE3822 | GEO

Mus musculus

Project description:RNA-SEQ analysis of single cells extracted from the renal vesicles of P4 Crym-EGFP transgenic mice

| PRJNA254484 | ENA

Transcription profiling of wild type and HoxA11 metenephric mesenchyme and ureteric bud from embryonic day 11.5 mice

Project description:E11.5 metanephric mesenchyme and ureteric bud were dissected from the E11.5 kidney rudiment using fine manual microdissection (ureteric bud only) or both fine manual microdissection and laser capture microdissection (metanephric mesenchyme) to define the gene expression profiles of these structures. Additionally, HoxA11, HoxD11 compound null E11.5 metanephric mesenchyme was obtained through laser capture microdissection allowing analysis of possible Hox targets in kidney development. Targets from multiple biological replicates of each were generated and the expression profiles were determined using Affymetrix MOE430_v2 arrays. Using microdissection techniques, ureteric bud and metanephric mesenchyme were dissected from E11.5 kidney rudiments allowing the identificated genes specifically regulated in either structure. In addition, Hoxa11, Hoxd11 compound null E11.5 metanephric mesenchyme were normalized to wild type embryonic controls allowing the identification of potential Hox targets in normal kidney development. Each structure/genotype were represented in biological (seperate embryo) replicate.

2007-12-07 | E-GEOD-3822 | biostudies-arrayexpress

Mus musculus

Project description:Gene expression profiles of E11.5 metanephric mesenchyme and Adult Juxtaglomerular Complex cells or renal cortex from the kidneys of Crym-GFP or Ren1c-YFP transgenic mice using FACS or single-cell isolation techniques (GUDMAP Series ID:47).

| PRJNA182921 | ENA

Mus musculus

Project description:Gene expression profiles of cap mesenchyme and renal vesicle isolated between P0-P4 from Crym-EGFP neonatal transgenic mice using FACS. (GUDMAP Series ID: 28)

| PRJNA123459 | ENA

RNA-SEQ analysis of single cells extracted from the metanephric mesenchyme of E11.5 Crym-EGFP transgenic mice

Project description:We used micro-dissection with FACS sorting techniques to isolate single cells from the metanephric mesenchyme of the Embryonic day 11.5 (E11.5) developing kidney. A subset of these single cell populations is analysed individually via Fluidigm single cell analysis. This analysis will determine the transcriptional profile of each cell type, identify compartment specific transcripts, compartment specific transcript isoforms and cell-type specific long-noncoding RNAs. In addition the unbiased nature of RNA-SEQ will potentially identify novel transcripts that have not been annotated in the database.

2014-08-01 | GSE59129 | GEO

Mus musculus

Project description:10.5dpc Metanephric mesenchyme Vs 11.5dpc metanephroi

| PRJNA91341 | ENA

Mus musculus

Project description:10.5dpc Metanephric Mesenchyme V Intermediate Mesoderm

| PRJNA91683 | ENA

15.5dpc Ureteric tree cells Vs rest

Project description:Triplicate pairwise comparsion of FACS sorted GFP+ve Vs GFP-ve cells from the kidneys of the HoxB7-GFP transgenic mice on compugen 22K mouse arrays. HoxB7-GFP mice express GFP in the ureteric tree and its derivatives while the metanephric mesenchyme, interstitium, developing vasculature etc do not.

2010-06-09 | E-GEOD-2281 | biostudies-arrayexpress

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