Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

ABSTRACT: Wolbachia endosymbiont of Drosophila recens (wRec) Genome sequencing

PROVIDER: PRJNA254527 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other
	SRR1508956_1.fastq.gz	Fastqsanger.gz
	SRR1508956_2.fastq.gz	Fastqsanger.gz

Items per page:

1 - 3 of 3

Similar Datasets

Tissue-Specific Transcriptomics and Proteomics of a Filarial Nematode and its Wolbachia Endosymbiont

Project description:We sequenced total RNA from Dirofilaria immitis in order to generate the first tissue-specific gene expression profile of a filarial nematode and its Wolbachia endosymbiont.

2016-06-01 | GSE67894 | GEO

Profiling tissue-specific modulation of gene expression in response to insulin signalling in Drosophila

Project description:We investigated tissue-specific regulation of gene expression in a long lived Drosophila IIS mutant (dilp2-3,5) compared to its control (wDah). As in the majority of Drosophila laboratory strains, the endosymbiont Wolbachia was present; in the absence of Wolbachia, life extension through dilp2-3,5 is abrogated (Grönke et al. 2010). To control for this, we additionally included strains of the same genotypes that lacked Wolbachia (dilp2-3,5T, wDahT). We quantified gene expression concurrently on the level of the proteome and the transcriptome, in four key tissues: brain, gut, fat body, and muscle. Proteome quantification was carried out on five biological replicates per experimental group. Corresponding transcriptome quantification was carried out on three biological replicates per experimental group, and published on GEO (accession number *GSE122190*)

2021-04-21 | PXD011589 | Pride

Wolbachia infection modifies the profile, shuttling and structure of microRNAs in a mosquito cell line

Project description:We characterized the miRNA composition of the nucleus and the cytoplasm of uninfected cells and compared it with the one of cells infected with the endosymbiotic bacterium Wolbachia strain wMelPop-CLA. We found an overall increase of small RNAs between 18 and 28 nucleotides in both cellular compartments in Wolbachia-infected cells and identified specific miRNAs induced and/or suppressed by the Wolbachia infection. We discuss the mechanisms that the cell may use to shuttle miRNAs between the cytoplasm and the nucleus. In addition, we identified piRNAs that changed their abundance in response to Wolbachia infection. The miRNAs and piRNAs identified in this study provide promising leads for investigations into the host-endosymbiont interactions and for better understanding of how Wolbachia manipulates the host miRNA machinery in order to facilitate its persistent replication in infected cells.

2014-02-22 | GSE55210 | GEO

Wolbachia mCGH of Five Supergroup A Strains

Project description:Using microarray-based comparative genome hybridizations (mCGH), the genomic content of Wolbachia pipientis wMel from Drosophila melanogaster was compared to the Wolbachia from D. innubila (wInn), D. santomea (wSan), and three strains from D. simulans (wAu, wRi, wSim).

2009-05-11 | GSE13825 | GEO

The relative importance of DNA methylation and Dnmt2-mediated epigenetic regulation on Wolbachia densities and cytoplasmic incompatibility

Project description:Wolbachia pipientis is a worldwide bacterial parasite of arthropods that infects host germline cells and manipulates host reproduction to increase the ratio of infected females, the transmitting sex of the bacteria. The most common reproductive manipulation, cytoplasmic incompatibility (CI), is expressed as embryonic death in crosses between infected males and uninfected females. Specifically, Wolbachia modify developing sperm in the testes by unknown means to cause a post-fertilization disruption of the sperm chromatin that incapacitates the first mitosis of the embryo. As these Wolbachia-induced changes are stable, reversible, and affect the host cell cycle machinery including DNA replication and chromosome segregation, we hypothesized that the host methylation pathway is targeted for modulation during cytoplasmic incompatibility because it accounts for all of these traits. Here we show that infection of the testes is associated with a 55% increase of host DNA methylation in Drosophila melanogaster, but methylation of the paternal genome does not correlate with penetrance of CI. Overexpression and knock out of the Drosophila DNA methyltransferase Dnmt2 neither induces nor increases cytoplasmic incompatibility. Instead, overexpression decreases Wolbachia titers in host testes by approximately 17%, leading to a similar reduction in CI levels. Finally, strength of CI induced by several different strains of Wolbachia does not correlate with levels of DNA methylation in the host testes. We conclude that DNA methylation mediated by Drosophila's only known methyltransferase is not required for the transgenerational sperm modification that causes CI. Genomic DNA was extracted from pooled samples of Drosophila melanogaster adult testes. One sample from Wolbachia-infected males and one from uninfected males. Bisulfite sequencing was used to determine whether Wolbachia infection affects host DNA methylation in the testes.

2014-12-03 | E-GEOD-63795 | biostudies-arrayexpress

Tissue-Specific Transcriptomics and Proteomics of a Filarial Nematode and its Wolbachia Endosymbiont

Project description:We sequenced total RNA from Dirofilaria immitis in order to generate the first tissue-specific gene expression profile of a filarial nematode and its Wolbachia endosymbiont. Examination of transcript levels in 7 different Dirofilaria immitis tissues, in duplicate, using Illumina GAIIx.

2016-06-01 | E-GEOD-67894 | biostudies-arrayexpress

Wolbachia endosymbiont of Drosophila subpulchrella strain:wSpc

Project description:Wolbachia endosymbiont of Drosophila subpulchrella

| PRJNA401169 | ENA

Wolbachia endosymbiont of Drosophila simulans wHa

Project description:Wolbachia endosymbiont of Drosophila simulans wHa Genome sequencing

| PRJNA176303 | ENA

Wolbachia endosymbiont of Drosophila simulans wNo

Project description:Wolbachia endosymbiont of Drosophila simulans wNo Genome sequencing

| PRJNA176302 | ENA

O. volvulus and Wolbachia Stage-specifc proteomes

Project description:Stage-specific proteomes of Onchocerca volvulus and its endosymbiont Wolbachia