Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Drosophila melanogaster

ABSTRACT: A strand-specific switch in noncoding transcription switches the function of a Polycomb/Trithorax response element

PROVIDER: PRJNA254705 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR1508950.fastq.gz	Fastqsanger.gz
	SRR1508951.fastq.gz	Fastqsanger.gz
	SRR1508952.fastq.gz	Fastqsanger.gz
	SRR1508953.fastq.gz	Fastqsanger.gz
	SRR1508954.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 6

Similar Datasets

A strand-specific switch in noncoding transcription switches the function of a Polycomb/Trithorax response element

Project description:Polycomb/Trithorax response elements (PRE/TREs) can switch their function reversibly between silencing and activation, by mechanisms that are poorly understood. Here we show that a switch in forward and reverse noncoding transcription from the Drosophila vestigial (vg) PRE/TRE switches the status of the element between silencing (induced by the forward strand) and activation (induced by the reverse strand). In vitro, both ncRNAs inhibit PRC2 histone methyltransferase activity, but in vivo only the reverse strand binds PRC2. Overexpression of the reverse strand evicts PRC2 from chromatin and inhibits its enzymatic activity. We propose that interactions of RNAs with PRC2 are differentially regulated in vivo, allowing regulated inhibition of local PRC2 activity. Genome-wide analysis shows that strand switching of ncRNAs occurs at several hundred PcG binding sites in fly and vertebrate genomes. This work identifies a novel and potentially widespread class of PRE/TREs that switch function by switching the direction of ncRNA transcription. E(Z) and H3K27me3 profile in Drosophila embryos of two transgenic lines (pKC27.vg.fwd' and pKC27.vg.rev') that were crossed to daGAL4 in order to overexpress the forward or the reverse vg PRE/TRE ncRNA from an ectopic site ('site 1' in this study).

2014-08-10 | E-GEOD-59192 | biostudies-arrayexpress

A strand-specific switch in noncoding transcription switches the function of a Polycomb/Trithorax response element

2014-08-10 | GSE59192 | GEO

Drosophila melanogaster

Project description:Comparing active and repressed expression states of genes controlled by the Polycomb/Trithorax group proteins

| PRJNA98389 | ENA

Chromatin maps of the Drosophila melanogaster TrxG protein Ash1 and FSH

Project description:Trithorax group (TrxG) proteins counteract Polycomb silencing by an as yet uncharacterized mechanism. A well-known member of the TrxG is the histone methyltransferase Absent, Small, or Homeotic discs 1 (ASH1). In Drosophila ASH1 is needed for the maintenance of Hox gene expression throughout development, which is tightly coupled to preservation of cell identity. In order to understand the molecular function of ASH1 in this process, we performed affinity purification of tandem-tagged ASH1 followed by mass spectrometry (AP-MS) and identified FSH, another member of the TrxG as interaction partner. Here we provide genome-wide chromatin maps of both proteins based on ChIP-seq.

2013-06-30 | GSE30820 | GEO

Chromatin maps of the Drosophila melanogaster TrxG protein Ash1 and FSH

2013-06-30 | E-GEOD-30820 | biostudies-arrayexpress

Drosophila melanogaster

Project description:Mrg15 allosterically activates Ash1’s H3K36 methyltransferase activity and facilitates Ash1’s trithorax group protein function in Drosophila

| PRJNA360011 | ENA

Drosophila melanogaster

Project description:New partners in regulation of gene expression: the Enhancer of Trithorax and Polycomb Corto interacts with methylated Ribosomal Protein L12 via its chromodomain.

| PRJNA167951 | ENA

Drosophila melanogaster

Project description:Chromosome conformation capture on chip (4C) at the Fab7 polycomb response element in the bithorax complex of Drosophila

| PRJNA130453 | ENA

Drosophila melanogaster

Project description:Chromosomal clustering of genes in trithorax (trx) mutant larvae

| PRJNA105287 | ENA

Drosophila melanogaster

Project description:Domain ChIRP reveals the modularity of long noncoding RNA architecture, function, and target genes

| PRJNA230684 | ENA