Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Caenorhabditis elegans

ABSTRACT: A Ribonuclease Coordinates siRNA Amplification and mRNA Cleavage during RNAi

PROVIDER: PRJNA255110 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR1513650.fastq.gz	Fastqsanger.gz
	SRR1513660.fastq.gz	Fastqsanger.gz
	SRR1513670.fastq.gz	Fastqsanger.gz
	SRR1513680.fastq.gz	Fastqsanger.gz
	SRR1513651.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 33

Similar Datasets

Caenorhabditis elegans

Project description:The Caenorhabditis elegans RDE-10/RDE-11 complex regulates RNAi by promoting secondary siRNA amplification

| PRJNA158125 | ENA

Caenorhabditis elegans

Project description:Analysis of 22G siRNA triggered siRNA amplification in Caenorhabditis elegans

| PRJNA156415 | ENA

The DEAD box helicase RDE-12 promotes amplification of RNAi in cytoplasmic foci in C. elegans

Project description:RNA interference (RNAi) is a potent mechanism for down-regulating gene expression. Conserved RNAi pathway components are found in animals, plants, fungi and other eukaryotes. In C. elegans, the RNAi response is greatly amplified by the synthesis of abundant secondary siRNAs. Exogenous double stranded RNA is processed by Dicer and RDE-1/Argonaute into primary siRNA that guides target mRNA recognition. The RDE-10/RDE-11 complex and the RNA dependent RNA polymerase RRF-1 then engage the target mRNA for secondary siRNA synthesis. However, the molecular link between primary siRNA production and secondary siRNA synthesis remains largely unknown. Furthermore, it is unclear if the sub-cellular sites for target mRNA recognition and degradation coincide with sites where siRNA synthesis and amplification occur. In the C. elegans germline, cytoplasmic P granules at the nuclear pores and perinuclear Mutator foci contribute to target mRNA surveillance and siRNA amplification, respectively. We report that RDE-12, a conserved FG domain containing DEAD-box helicase, localizes in P-granules and cytoplasmic foci that are enriched in RSD-6 but are excluded from the Mutator foci. Our results suggest that RDE-12 promotes secondary siRNA synthesis by orchestrating the recruitment of RDE-10 and RRF-1 to primary siRNA targeted mRNA in distinct cytoplasmic compartments.

2014-03-27 | GSE53830 | GEO

Analysis of 22G siRNA triggered siRNA amplification in Caenorhabditis elegans

Project description:To determine if an endogenous 22G siRNA sensor transgene is subject to siRNA amplification, small RNAs were deep sequenced from the sensor and from a control transgene that is identical to the sensor but lacks an siRNA target site.

2011-12-10 | GSE34320 | GEO

Caenorhabditis elegans

Project description:Distinct phases of siRNA synthesis in an endogenous RNAi pathway in C. elegans soma

| PRJNA121791 | ENA

Caenorhabditis elegans

Project description:mRNA expression analysis in mock and cgh-1(RNAi) animals

| PRJNA155455 | ENA

Caenorhabditis elegans

Project description:The DEAD box helicase RDE-12 promotes amplification of RNAi in cytoplasmic foci in C. elegans

| PRJNA233484 | ENA

Caenorhabditis elegans

Project description:Amplification of siRNA in Caenorhabditis elegans generates a transgenerational sequence-targeted histone H3 lysine 9 methylation footprint

| PRJNA146973 | ENA