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Genome-wide DNA methylation analysis reveals dynamic changes in the cardiac methylome during post-natal heart development

ABSTRACT: Genome-wide DNA methylation analysis reveals dynamic changes in the cardiac methylome during post-natal heart development

PROVIDER: PRJNA257194 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR1533761.fastq.gz	Fastqsanger.gz
	SRR1533762.fastq.gz	Fastqsanger.gz
	SRR1533763.fastq.gz	Fastqsanger.gz
	SRR1533754.fastq.gz	Fastqsanger.gz
	SRR1533764.fastq.gz	Fastqsanger.gz

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Genome-wide DNA methylation analysis reveals dynamic changes in the cardiac methylome during post-natal heart development (MBD-Seq)

Project description:Epigenetic modifications have emerged as central players in the coordination of gene expression networks during cardiac development. While several studies have investigated the role of histone modifications during heart development, relatively little is known about the role of DNA methylation. The purpose of the current study was to determine whether DNA methylation plays an important role in guiding transcriptional changes during the neonatal period, which is an important developmental window for cardiac maturation and cardiomyocyte cell cycle arrest. We used methyl binding domain protein sequencing (MBD-seq) and mRNA-seq to profile DNA methyation and gene expression respectively in neonatal hearts at P1 and P14 stages. Thousands of differentially methylated regions (DMRs) were identified between P1 and P14, the vast majority of which were hypermethylated. Gene ontology analysis revealed that these hypermethylated genes were associated with transcriptional regulation of important developmental signaling pathways, including Hedgehog, BMP, TGF beta, FGF and Wnt/b-catenin signaling. A significant enrichment for myogenic transcription factors and Smad2/3/4 binding sites was also noted among differentially methylated peaks at P14. This study provides novel evidence for widespread alterations in DNA methylation during post-natal heart maturation and suggests that DNA methylation plays an important role in cardiomyocyte cell cycle arrest during the neonatal period. We used methyl binding domain protein sequencing (MBD-seq) to profile DNA methyation in neonatal hearts at P1 and P14 stages (post-natal day 1 and 14 respectively) in three biological replicates.

2014-08-01 | E-GEOD-59969 | biostudies-arrayexpress

Genome-wide DNA methylation analysis reveals dynamic changes in the cardiac methylome during post-natal heart development (RNA-Seq)

Project description:Epigenetic modifications have emerged as central players in the coordination of gene expression networks during cardiac development. While several studies have investigated the role of histone modifications during heart development, relatively little is known about the role of DNA methylation. The purpose of the current study was to determine whether DNA methylation plays an important role in guiding transcriptional changes during the neonatal period, which is an important developmental window for cardiac maturation and cardiomyocyte cell cycle arrest. We used methyl binding domain protein sequencing (MBD-seq) and mRNA-seq to profile DNA methyation and gene expression respectively in neonatal hearts at P1 and P14 stages. Thousands of differentially methylated regions (DMRs) were identified between P1 and P14, the vast majority of which were hypermethylated. Gene ontology analysis revealed that these hypermethylated genes were associated with transcriptional regulation of important developmental signaling pathways, including Hedgehog, BMP, TGF beta, FGF and Wnt/b-catenin signaling. A significant enrichment for myogenic transcription factors and Smad2/3/4 binding sites was also noted among differentially methylated peaks at P14. This study provides novel evidence for widespread alterations in DNA methylation during post-natal heart maturation and suggests that DNA methylation plays an important role in cardiomyocyte cell cycle arrest during the neonatal period. mRNA-seq to profile gene expression in neonatal hearts at P1 and P14 stages (post-natal day 1 and 14 respectively) in three biological replicates.

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Genome-wide DNA methylation analysis reveals dynamic changes in the cardiac methylome during post-natal heart development (RNA-Seq)

2014-08-01 | GSE59970 | GEO

Genome-wide DNA methylation analysis reveals dynamic changes in the cardiac methylome during post-natal heart development (MBD-Seq)

2014-08-01 | GSE59969 | GEO

Genome-wide DNA methylation analysis reveals dynamic changes in the cardiac methylome during post-natal heart development

Project description:This SuperSeries is composed of the SubSeries listed below. Refer to individual Series

2014-08-01 | E-GEOD-59971 | biostudies-arrayexpress

Genome-wide DNA methylation analysis reveals dynamic changes in the cardiac methylome during post-natal heart development

Project description:This SuperSeries is composed of the SubSeries listed below.

2014-08-01 | GSE59971 | GEO

Expression data from pre natal and post natal CCAM samples and relative controls

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Expression data from post-natal rat Achilles tendons

Project description:We have compared the gene expression profile of post-natal 1 day and 7 day rat Achilles tendons.

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2021-12-02 | GSE189887 | GEO

Expression data from post-natal rat Achilles tendons

Project description:We have compared the gene expression profile of post-natal 1 day and 7 day rat Achilles tendons. Post-natal 1 day and 7 day rat Achilles tendons were collected. Each sample contains at least two individuals. Total RNA was extracted and fragmented biotin-tagged cRNA was hybridized to Rat Genome 230 2.0 Array.

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