Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Drosophila melanogaster

ABSTRACT: Drosophila melanogaster midgut transcriptome analysis after an oral infection by the entomopathogen bacteria Serratia marcescens

PROVIDER: PRJNA258425 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR1553350.fastq.gz	Fastqsanger.gz
	SRR1553351.fastq.gz	Fastqsanger.gz
	SRR1553352.fastq.gz	Fastqsanger.gz
	SRR1553353.fastq.gz	Fastqsanger.gz
	SRR1553348.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 11

Similar Datasets

Drosophila melanogaster midgut transcriptome analysis after an oral infection by the entomopathogen bacteria Serratia marcescens

Project description:Drosophila melanogaster oral infection by the entomopathogen bacteria Serratia marcescens trigger, at the midgut level, a drastic phenotype during the early phase of the exposure. In response to Serratia marcescens virulence factors the enterocytes present a rapid formation of megamitochondria and a subsequent controlled extrusion of the cytoplasm along with damaged organelles, which may constitute a repair mechanism. This results in a thin intestinal epithelium that then recovers its original shape in just a few hours. In order to identify, at the midgut level, the transcriptional modifications induced by Serratia marcescens during this early phase of the infection, we performed a RNAseq transcriptomics analysis of the flies intestine three hours after bacteria ingestion. We found that 144 genes were significantly induced and that 34 genes were repressed at this time point in comparison to the non infected midguts.

2016-11-22 | GSE60504 | GEO

Serratia_infections

Project description:Transcriptional responses in the gut of the main malaria vector Anopheles gambiae following oral bacterial infection with the entomopathogen Serratia marcescens were identified using DNA microarrays. S. marcescens is a common member of the mosquito gut microbiota, found in both laboratory reared and field collected mosquitoes, that can be potentially pathogenic as in Drosophila (Nehme et al., 2007), while it has been shown to influence the outcome of Plasmodium infections (Bando et al., 2013). S. marcescens belongs to the Enterobacteriaceae family, members of which have been shown to influence malaria transmission dynamics (Cirimotich et al., 2011, Boissiere et al., 2012). To further investigate the interactions between S. marcescens and the mosquito host, likely to shape, directly or indirectly, malaria transmission dynamics, An. gambiae mosquitoes, from the recently established N'gousso M form laboratory colony that retains much of the genetic variation of field mosquitoes, were antibiotic treated for 5 days and subsequently orally infected with the Db11-GFP strain of S. marcescens. Bacteria-fed mosquitoes were selected 2 days post infection, and, 3 days post infection, guts from bacteria-fed mosquitoes were dissected. Uninfected control mosquitoes were treated in the same way. Differential expression in the gut of S. marcescens infected mosquitoes, compared to uninfected controls, was identified by hybridizing labelled complementary RNA, derived from total RNA extracted from the respective gut pools, in customized Agilent 4x44k gene expression microarrays, comprising oligonucleotide probes encompassing all An. gambiae annotated genes of the AgamP3.6 release, with each probe represented in duplicate.

2013-11-30 | E-MEXP-3952 | biostudies-arrayexpress

Effects of Pseudomonas aeruginosa ATCC27853 ingestion on Drosophila melanogaster

Project description:Drosophila melanogaster is a validated eukaryotic model for immunity-concerned studies in the post-genomic era. In the present study we performed oral experimental infection of D. melanogaster with Pseudomonas aeruginosa (strain ATCC27853). By using a whole genome microarray approach, we intended to identify significant alterations in the expression profile of relevant genes amenable to qualify as new models for the investigation of specific host-parasite interactions.

2016-04-09 | GSE80084 | GEO

Drosophila melanogaster

Project description:Drosophila melanogaster Infection-regulated Transcriptome

| PRJNA348190 | ENA

Drosophila melanogaster

Project description:Host response after intestinal infection with Serratia marcescens Db11

| PRJNA821079 | ENA

Drosophila melanogaster

Project description:Development of Drosophila Midgut

| PRJNA958606 | ENA

Drosophila melanogaster

Project description:Drosophila melanogaster