Project description:Transcriptional profiling of Du145-PCAT1 and RWPE-PCAT1 cells treated with either non-targeting siRNA or two independent siRNAs targeting cMyc. The goal was to determine the role of cMyc in mediating PCAT-1 transcriptional regulation. Three-condition experiment, PCAT1 cells with non-targeting siRNA vs cMyc siRNA #3 or cMyc siRNA #4. Performed on two cell lines, Du145 and RWPE. Biological replicates: 2 control replicates per cell line, 2 transfected replicates per siRNA per cell line.
Project description:cMyc is a master regulator of transcription in growing cells. Here, we used microarray to study global transcriptomic expression of cMyc knockdown HT1080 cells to investigate cMyc- regulated gene expression.
Project description:Transcriptional profiling of Du145-PCAT1 and RWPE-PCAT1 cells treated with either non-targeting siRNA or two independent siRNAs targeting cMyc. The goal was to determine the role of cMyc in mediating PCAT-1 transcriptional regulation.
Project description:Ubiquitylation proteomics experiment for HEK293T cells treated with either non-targeting shRNA, or shRNA knockdown of AMBRA1 by two independent shRNA's (shAMBRA1-TRC20, and shAMBRA1-TRC36).
Project description:RNAseq of primary human erythroid progenitor cells expressing a RHEX targeting shRNA vs. a non-targeting control shRNA NT (luciferase shRNA): Insight into RHEX's pro-erythropoietic effects
Project description:We have evaluated the microRNA expression profile of SUM159 cells stably infected with a control shRNA or a dicer-targeting shRNA treated with vehicle or metformin for 24 hrs at non cytotoxic doses.
Project description:We have evaluated the microRNA expression profile of SUM159 cells stably infected with a control shRNA or a dicer-targeting shRNA treated with vehicle or metformin for 24 hrs at non cytotoxic doses. microRNA expression was evaluated from total RNA extracted from logaritmically growing SUM159 cells stably expressing a control shRNA or a dicer-targeting shRNA and treated with vehicle (PBS) or metformin (0.5mM) for 24 hrs.
