Project description:Transcriptome of A. nidulans ∆pkaA strain when grown on complete media (CM) and transferred to minimal media plus avicel as a sole carbon source for 8 and 24 hours
Project description:Transcriptome of A. nidulans TNO2a3, ∆snfA and ∆schA strains when grown on complete media (CM) and transferred to minimal media plus avicel as a sole carbon source for 8 and 24 hours.
Project description:Transcriptome of A. nidulans TNO2A3, ∆msbA and ∆MHD strains when grown on complete media (YUU) and transferred to minimal media plus avicel as a sole carbon source for 24 hours
Project description:Transcriptome of A. nidulans TNO2A3, M-bM-^HM-^FmsbA and M-bM-^HM-^FMHD strains when grown on complete media (YUU) and transferred to minimal media plus avicel as a sole carbon source for 24 hours Two conditions: complete media (reference) for 24h and minimal media plus avicel for 24 hours. Three strains TNO2A3, M-bM-^HM-^FmsbA and M-bM-^HM-^FMHD. Three biological repetitions of each point.
Project description:Transcriptome of A. nidulans TNO2a3, M-bM-^HM-^FsnfA and M-bM-^HM-^FschA strains when grown on complete media (CM) and transferred to minimal media plus avicel as a sole carbon source for 8 and 24 hours. Three conditions: complete media (reference) for 24h, and minimal media plus avicel for 8 and 24 hours. Three strains: TNO2a3, M-bM-^HM-^FsnfA and M-bM-^HM-^FschA. Three biological repetitions of each timepoint of TNO2a3 and M-bM-^HM-^FschA, and two for each timepoint of M-bM-^HM-^FsnfA.
Project description:Transcriptome of A. nidulans TNO2a3 and ∆ptpB strains when grown on minimal media plus casaminoacids and transferred to minimal media plus glucose as a sole carbon source for 4 hours
Project description:Purpose: To explore conservation of gene regulation by the transcription factor clr-2/clrB in Neurospora crassa and Aspergillus nidulans Methods: mRNA from wild type and clr-2/clrB mutants were collected after a culture shift from sucrose/glucose to Avicel (crystaline cellulose) or no carbon media Results: We show that N. crassa and A. nidulans have similair global transcriptional responses to Avicel, with several hundred genes showing specific induction, though the induced genes are more specifically targeted at cellulose for N. crassa and more targeted at hemicellulose and pectin for A. nidulans. clr-2/clrB has a conserved fundamental function in cellulose induction, though the mechanism has diverged. Misexpression of clr-2 is sufficeint for inducer free cellulase secretion in N. crassa, but neither clrB or heterologous clr-2 is sufficient for inducer free cellulase secretion in A. nidulans. Conclusions: Our study demonstrates a conserved and essential role in cellulose utilization for the transcription factor clr-2 in filamentous ascomycetes and demonstrates that manipulation of clr-2 expression can be used to control cellulase expression in some species.
Project description:Transcriptome of A. nidulans TNO2a3 and M-bM-^HM-^FptpB strains when grown on minimal media plus casaminoacids and transferred to minimal media plus glucose as a sole carbon source for 4 hours Three conditions minimal media plus casaminoacids during 24 hours (reference) and minimal media plus glucose for 4 hours. Three strains TNO2a3 and M-bM-^HM-^FptpB. Three biological repetitions of each timepoint of TNO2a3 / M-bM-^HM-^FptpB
