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Astyanax mexicanus

ABSTRACT: Astyanax mexicanus - Developmental Gene Expression

PROVIDER: PRJNA258661 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR1556300.fastq.gz	Fastqsanger.gz
	SRR1556201.fastq.gz	Fastqsanger.gz
	SRR1556301.fastq.gz	Fastqsanger.gz
	SRR1556193.fastq.gz	Fastqsanger.gz
	SRR1556273.fastq.gz	Fastqsanger.gz

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RNA-Seq Analysis of liver cells of Astyanax mexicanus.

Project description:Organisms adapt to and survive in environments with varying nutrient availability. Cis-regulatory changes play important roles in adaptation and phenotypic evolution. To what extent cis-regulatory elements contribute to metabolic adaptation is less understood. Here we have utilized a unique vertebrate model, Astyanax mexicanus, that survives in nutrient rich surface and nutrient deprived cave water to uncover gene regulatory networks in metabolic adaptation. We performed genome-wide analysis of accessible chromatin and histone modifications in the liver tissue of one surface and two independently derived cave populations, providing the first genome-wide epigenetic landscape in this organism. In parallel, we performed RNA Seq as a read out of gene expression. We find that many cis-regulatory elements differ between surface and the cavefish, while the two independently derived cave populations have evolved remarkably similar regulatory signatures. Changes in gene regulatory networks between the surface and cave morphotypes point to global changes in key metabolic pathways.

2021-09-07 | GSE153050 | GEO

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