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DNA methylation at transcription start sites blocks TBP binding and transcription onset by RNApolII

ABSTRACT: DNA methylation at transcription start sites blocks TBP binding and transcription onset by RNApolII

PROVIDER: PRJNA259204 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR1556196_1.fastq.gz	Fastqsanger.gz
	SRR1556196_2.fastq.gz	Fastqsanger.gz
	SRR1556197_1.fastq.gz	Fastqsanger.gz
	SRR1556197_2.fastq.gz	Fastqsanger.gz

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Project description:DNA methylation at transcription start sites blocks TBP binding and transcription onset by RNApolII [ChIPbisSeq]

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DNA methylation at transcription start sites blocks TBP binding and transcription onset by RNApolII

Project description:This SuperSeries is composed of the SubSeries listed below.

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DNA methylation at transcription start sites blocks TBP binding and transcription onset by RNApolII [MeDIP]

Project description:We measured the methylation levels of gene promoters of cerebral cortex of mice. We used MeDIP-array, immunoprecipitating genomic DNA with an antibody against methylated DNA and measuring methylation levels using custom arrays. The input DNA was used as control.

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DNA methylation at transcription start sites blocks TBP binding and transcription onset by RNApolII [ChIPbisSeq]

Project description:We report the application of ChIP bisulfite sequencing (ChIPbisSeq) to establish the methylation state of DNA bound to RNApol2 phosphorylated in Ser5 (RNAPol2Ser5) in the mouse cortex. We first profiled the RNAPol2Ser5 binding using regular ChIPSeq from 45 million raw read pairs (31 million unique pairs were aligned to the genome). Then we used bisulfite converted DNA immunoprecipitated with an antibody against RNAPol2Ser5 (87 million raw read pairs, 38 million unique read pairs aligned to the genome) to map RNAPol2Ser5 sites and to establish the methylation state at these sites.

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Architecture of TAF11/TAF13/TBP complex suggests novel regulatory state of human general transcription factor TFIID

Project description:General transcription factor TFIID is a key component of RNA polymerase II transcription initiation in eukaryotic nuclei. Human TFIID is a megadalton-sized multiprotein complex comprising TATA-binding protein (TBP) and 13 TBP-associated factors (TAFs). TBP binds to core promoter DNA, recognizing the TATA-box. A number of transcription regulatory factors were found to compete with DNA for TBP binding. We identified a ternary complex formed by TBP and the histone fold (HF) domain containing TFIID subunits TAF11 and TAF13. We demonstrate that TAF11/TAF13 competes for TBP binding with TATA-box DNA, and also with the N-terminal domain of TAF1. In an integrative approach combing crystal coordinates, biochemical analyses and data from cross-linking mass-spectrometry (CLMS), we determine the architecture of the TAF11/TAF13/TBP complex, revealing TAF11/TAF13 interaction with the DNA binding surface of TBP. Our results thus suggest a novel regulatory state for TFIID function.

2017-11-15 | PXD005676 | Pride