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Pyronema omphalodes CBS 100304

ABSTRACT: Pyronema confluens circadian rhythm analysis

PROVIDER: PRJNA260620 | ENA |

REPOSITORIES: ENA

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Dataset's files

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			Action	DRS
	SRR1571980.fastq.gz	Fastqsanger.gz
	SRR1571981.fastq.gz	Fastqsanger.gz
	SRR1571982.fastq.gz	Fastqsanger.gz
	SRR1571977.fastq.gz	Fastqsanger.gz
	SRR1571978.fastq.gz	Fastqsanger.gz

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Pyronema omphalodes CBS 100304

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Gene expression in protoapothecia from Pyronema confluens

Project description:The aim of this analysis was to analyze gene expression in young fruiting bodies of the filamentous ascomycete Pyronema confluens. Young fruiting bodies were isolated by laser microdissection, RNA was extracted, amplified and used for RNA-sequencing. The resulting data were compared to data from total vegetative and sexual mycelium from a previous analysis.

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Project description:This transcriptome analysis accompanies the genome sequencing of Pyronema confluens for purposes of annotation and gene expression quantification. P. confluens is a basal filamentous ascomycete that forms primitive fruiting bodies (apothecia) to generate its sexual spores. We have performed RNA-seq for two growth conditions allowing only vegetative growth and one condition allowing sexual development to identify genes that are differentially regulated during fruiting body formation. The samples sex (sexual development), DD (growth in darkness, no sexual development), and vegmix (pool of several conditions, no sexual development) were sequenced as paired-end reads on an Illumina HiSeq 2000, two independent biological replicates for each sample, two technical replicates for each biological replicate (each library was sequenced twice in two independent flow cells).

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