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Saccharomyces cerevisiae

ABSTRACT: Transcription profile of phm3 damp strain during growth in no phosphate medium

PROVIDER: PRJNA261794 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR1583860.fastq.gz	Fastqsanger.gz
	SRR1583870.fastq.gz	Fastqsanger.gz
	SRR1583880.fastq.gz	Fastqsanger.gz
	SRR1583861.fastq.gz	Fastqsanger.gz
	SRR1583871.fastq.gz	Fastqsanger.gz

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Expression data from Saccharomyces cerevisiae and Saccharomyces pastorianus var. carlsbergensis

Project description:During fermentation Saccharomyces yeast produces various aroma-active metabolites determining the different characteristics of aroma and taste in fermented beverages. Amino acid utilization by yeast during brewer´s wort fermentation is seen as linked to flavour profile. To better understand the relationship between the biosynthesis of aroma relevant metabolites and the importance of amino acids, DNA microarrays were performed for Saccharomyces cerevisiae strain S81 and Saccharomyces pastorianus var. carlsbergensis strain S23, respectively. Thereby, changes in transcription of genes were measured, which are associated with amino acid assimilation and its derived aroma-active compounds during fermentation.

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Project description:Arsenic is known as a human carcinogen that easily be exposed by the living organisms through environment and food consumption. The arsenic is transport into the cells via phosphate transporters due to its structural similarity with phosphate in both prokaryotes and eukaryotes. We here evaluated and analyzed the toxicogenomic impacts of arsenate and the role of different phosphate concentrations on arsenic toxicity. Our results showed that arsenic uncoupled phosphate levels which eventually affected the growth rate of yeast cells. Analysis of arsenate levels in the medium over 4 to 10 h of its exposure clearly showed that arsenate was easily taken up by the cells in phosphate limited condition.

2009-12-31 | GSE14991 | GEO

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