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Solanum tuberosum

ABSTRACT: Transcriptional changes in dormant and non_dormant potato meristems

OTHER RELATED OMICS DATASETS IN: E-GEOD-61690

PROVIDER: PRJNA261838 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR1584270.fastq.gz	Fastqsanger.gz
	SRR1584263.fastq.gz	Fastqsanger.gz
	SRR1584264.fastq.gz	Fastqsanger.gz
	SRR1584265.fastq.gz	Fastqsanger.gz
	SRR1584266.fastq.gz	Fastqsanger.gz

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Project description:Transcriptional changes in dormant potato minitubers treated with 1-(alpha-ethylbenzy)-3-nitroquanidine

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Project description:Gene Expression Changes In potato Tuber Meristems Treated with the Sprout Inhibitor 1,4-dimethylnapthlene

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E-GEOD-61690

Project description:Transcriptional changes in dormant and non_dormant potato meristems

2016-04-07 | E-GEOD-61690 | ExpressionAtlas

Transcriptome analysis of dormant and sprouting bud tissue from potato (Solanum tuberosum L.)

Project description:Samples from dormant and sprouting bud tissue from potato tubers were taken to enable global transcriptome analysis of active meristematic tissue. Data was used in a co-expression analysis using microarray data of different experimental backgrounds.

2016-12-12 | E-MTAB-4806 | biostudies-arrayexpress

Transcriptional changes in dormant and non_dormant potato meristems

Project description:RNA was sequenced from meristems excised from dormant and non-dormant potato tubers harvested from four different harvest years.

2014-09-24 | GSE61690 | GEO

Transcription profiling of Solanum tuberosum leaves in a diurnal time course

Project description:Transcriptional changes in potato leaves over a diurnal period

2010-02-08 | E-MEXP-2481 | biostudies-arrayexpress

Transcriptional changes in dormant and non_dormant potato meristems

Project description:RNA was sequenced from meristems excised from dormant and non-dormant potato tubers harvested from four different harvest years. Expression based on mapped RNA-sequences was accomplished from excised meristems from fall harvested (dormant tubers) and the same harvested tubers were stored under standard commercial conditions until sprouting was present (non-dormant). The experiment was replicated for four different harvest years.

2014-09-24 | E-GEOD-61690 | biostudies-arrayexpress

Integrated RNA-seq and sRNA-seq analysis reveals miRNA effects on secondary metabolism in Solanum tuberosum L [miRNA-seq]

Project description:Light is a major environmental factor that affects metabolic pathways and stimulates the production of secondary metabolites in potato. However, adaptive changes in potato metabolic pathways and physiological functions triggered by light are partly explained by gene expression changes. Regulation of secondary metabolic pathways in potato has been extensively studied at transcriptional level, but little is known about the mechanisms of post-transcriptional regulation by miRNAs. To identify light-responsive miRNAs/mRNAs and construct putative metabolism pathways regulated by the miRNA-mRNA pairs, an integrated omics (sRNAome and transcriptome) analysis was performed to potato under light stimulus. A total of 31 and 48 miRNAs were identified to be differentially expressed in the leaves and tubers, respectively. Among the DEGs, 1353 genes in the leaves and 1841 genes in the tubers were upregulated, while 1595 genes in the leaves and 897 genes in the tubers were downregulated by light. Mapman enrichment analyses showed that genes related to MVA pathway, alkaloids-like, phenlypropanoids, flavonoids, and carotenoids metabolism were significantly upregulated, while genes associated with major CHO metabolism were repressed in the leaves and tubers. Integrated miRNA and mRNA profiles revealed that light-responsive miRNAs are important regulators in alkaloids metabolism, UMP-salvage, lipid biosynthesis, and cellulose catabolism. Moreover, several miRNAs may participate in glycoalkaloids metabolism via JA signaling pathway, UDP-glucose biosynthesis and hydroxylation reaction. This study provides a global view of transcriptome response in potato response to light, our results suggest that miRNAs might play important roles in secondary metabolic pathways, especially in glycoalkaloid biosynthesis. The findings will enlighten us on the genetic regulation of secondary metabolite pathways and pave the way for future application of genetically engineered potato.

2016-09-28 | GSE79984 | GEO

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Project description:Transcriptional profiling of two stress responses in potato

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PLRV co-immunoprecipitation from Solanum tuberosum

Project description:Phloem localization of plant viruses is advantageous for acquisition by sap-sucking vectors but hampers host-virus protein interaction studies. In this study, Potato leafroll virus (PLRV)-host protein complexes were isolated from systemically infected potato, a natural host of the virus. Comparing two different co-immunoprecipitation support matrices coupled to mass spectrometry, we identified 44 potato proteins and one viral protein (P1) specifically associated with virus isolated from infected phloem. An additional 142 proteins interact in complex with virus at varying degrees of confidence. Greater than 80% of these proteins were previously found to form high confidence interactions with PLRV isolated from the model host Nicotiana benthamiana. Bioinformatics revealed that these proteins are enriched for functions related to plasmodesmata, organelle membrane transport, translation and mRNA processing. Our results show that model system proteomics experiments are extremely valuable for understanding protein interactions regulating infection in recalcitrant pathogens such as phloem-limited viruses.

2016-12-14 | PXD004814 | Pride

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