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Escherichia coli REL606

ABSTRACT: Escherichia coli Long-term Evolution Experiment 500 and 1000 Generation Population Sequencing

PROVIDER: PRJNA262100 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR1586210_1.fastq.gz	Fastqsanger.gz
	SRR1586210_2.fastq.gz	Fastqsanger.gz
	SRR1586220_1.fastq.gz	Fastqsanger.gz
	SRR1586220_2.fastq.gz	Fastqsanger.gz
	SRR1586201_1.fastq.gz	Fastqsanger.gz

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Evolution of gene expression during long term coexistence in a bacterial evolution experiment

Project description:We used microarrays to study the evolution of gene expression in two bacterial ecotypes that coexisted for more than 35000 generations of experimental evolution in an extremely simple environment We sampled four clones from each of two lineages at generations 6,500, 17,000 and 40,000 from the polymorphic Ara-2 population of the E. coli long-term evolution experiment. The four clones from each lineage and generation were mixed equally and used for global expression profiling, growth assays and competition experiments. Global expression profiles and growth assays were also performed on the ancestral strain. RNA extractions were done using cells in mid-exponential growth in the same glucose-limited minimal medium used in the evolution experiment. Global expression profiles were obtained by using Affymetrix arrays, with 5 or 6 biological replicates for each lineage-generation sample.

2012-01-01 | E-GEOD-30639 | biostudies-arrayexpress

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