Project description:Human prostate cancer cell line LNCaP was stably transfected with a construct expressing shRNA against CRISP-3 gene. Knockdown of CRISP-3 was confirmed at RNA and protein level in the selected stable clones at three different passages. In order to identify the possible signalling pathways regulated by CRISP-3, one of the knockdown clones was subjected to microarray analysis. Global gene expression profile of the CRISP-3 knockdown clone was compared to that of the emprty vector clone. It was observed that Kallikrein 3 was significantly downregulated upon CRISP-3 knockdown whereas Annexin A1 and Vimentin were one of the highly upregulated genes.
Project description:Analysis of gene expresssion altered upon knockdown of histone demethylase JMJD1A in human prostate cancer cells. The objective is to elucidate the transcriptional programs that are controlled by JMJD1A in human prostate cancer. CWR22Rv1 prostate cancer cells were transduced with lentiviral particles encoding control pLKO.1 or JMJD1A shRNA (shJMJD1A). After 48 h, total RNAs were collected for the microarray analysis to determine the differentially expressed genes between Rv1 pLKO.1 and Rv1 shJMJD1A samples.
Project description:To identify genes and pathways downstream of Canonical WNT signaling in prostate cancer, we performed RNA sequencing to capture the transcriptional changes upon APC knockdown or RNF43 knockdown plus Wnt3a stimulation in VCaP cells. The global changes of gene expression were assessed by comparing APC knockdown with Control knockdown or RNF43 knockdown plus Wnt3a stimulation with RNF43 knockdown. By overlapping the genes differentially expressed under both conditions, we obtained a bona fide WNT/β catenin downstream effectors list in prostate cancer.
2022-03-01 | GSE188557 | GEO
Project description:To determine the differentially expressed genes in cancer associated fibroblasts upon knockdown of ACLP.
