Project description:Gene expression profiling of immortalized human mesenchymal stem cells with hTERT/E6/E7 transfected MSCs. hTERT may change gene expression in MSCs. Goal was to determine the gene expressions of immortalized MSCs.
Project description:Transcriptional profiling of human mesenchymal stem cells comparing normoxic MSCs cells with hypoxic MSCs cells. Hypoxia may inhibit senescence of MSCs during expansion. Goal was to determine the effects of hypoxia on global MSCs gene expression.
Project description:The aim of this study was to identify molecular targets for FGF-8 associated with prostate cancer progression in PC3 prostate cancer cells. PC3 prostate cancer cells were stably transfected with FGF-8b or control vector (mock). Expression of FGF-8 in PC3 cells increased cell growth and MMP-production in vitro. Microarray analyses revealed 169 significantly altered genes (>2.0-fold), some of the which were known to be associated with cancer growth and may represent novel markers of FGF-8 function. Upregulated genes included those involved in angiogenesis or cell growth (DDAH2, CRIP1 and SHC). Genes associated with differentiation or cell death were downregulated (VDR, CYCS). This study demonstrates associated patterns of gene expressions for FGF-8 in prostate cancer. This analysis may help find new prognostic factors or therapeutic targets for prostate cancer.
