Project description:Although the majority of genomic binding sites for the insulator protein CTCF are constitutively occupied, a subset show variable occupancy. Such variable sites provide an opportunity to assess context-specific CTCF functions in gene regulation. Here we have identified a variably occupied CTCF site in the Ultrabithorax (Ubx) gene in Drosophila. This site is occupied in tissues where Ubx is active (third thoracic imaginal leg disc) but is not bound in tissues where the Ubx gene is repressed (first thoracic imaginal leg disc). Comparison of CTCF binding in T1 leg disc vs T3 leg disc in from 3rd instar larva
Project description:Transcriptomes of Drosophila melanogaster eye-antennal imaginal discs at three sequential larval stages: late 2nd instar (72h after egg laying (AEL)), mid 3rd instar (96h AEL) and late 3rd instar (120h AEL).
Project description:Study of genomic distribution of H3K27me3 in Drosophila melanogaster Myb mutant wing discs of 3rd instar larvae compared to control animals.
Project description:Study of genomic distribution of H3K4me3 in Drosophila melanogaster Myb mutant wing discs of 3rd instar larvae compared to control animals.
Project description:We report on changes in gene expression resulting from the ecdysone-directed broad-based transcriptional regulatory hierarchy in Drosophila melanogaster leg imaginal discs by comparing expression in white1118 third-instar larvae (-18 hrs relative to pupariation) and white prepupae (0 hrs) to examine the transcriptional effects of the late larval ecdysone pulse, as well comparing expression in white1118 and broad5 white prepupal leg imaginal discs to examine the transcriptional effects of the early ecdysone responder, broad. Using this approach, we were able to identify critical leg disc elongation genes that are differentially regulated in broad mutants
Project description:Study of genome-wide differential gene expression of Drosophila Melanogaster Myb mutant wing discs of 3rd instar larvae compared to control animals.
Project description:Study of genome-wide differential gene expression of Drosophila Melanogaster Myb mutant wing discs of 3rd instar larvae compared to control animals.
Project description:Study of genomic distribution of RNA polymerase in Drosophila melanogaster Myb mutant wing discs of 3rd instar larvae compared to control animals.
