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Vitis vinifera

ABSTRACT: Transcriptomic analysis of grape (Vitis vinifera L.) leaves exposed to ultraviolet C radiation

OTHER RELATED OMICS DATASETS IN: E-GEOD-62315

PROVIDER: PRJNA263817 | ENA |

REPOSITORIES: ENA

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Transcriptomic analysis of grape (Vitis vinifera L.) leaves exposed to ultraviolet C radiation

2014-11-15 | GSE62315 | GEO

Transcriptomic analysis of grape (Vitis vinifera L.) leaves exposed to ultraviolet C radiation

Project description:Solar ultraviolet C（UV-C）radiation reaching the Earth’s surface is little due to the filtering effects of the stratospheric ozone layer. At present, artificial UV-C irradiation is utilized for different biological processes. Grape is a major fruit crop around the world. Research has shown that UV-C irradiation induced the biosynthesis of phenols. However, changes at the molecular level in response to UV-C and leading to these effects are poorly understood. To elucidate the effect of UV-C on expression of genes in grape and the response mechanism, transcript abundance of grape (Vitis vinifera L.) leaves was quantified using the Affymetrix Grape Genome oligonucleotide microarray (15,700 transcripts) Grape leaves were exposed to UV-C irradiation at 6W/m2 for 10 min. LCK-0-1, LCK-0-2 and LCK-0-3 are 0 h after the initiation of treatment and as the controls; LTR-6-1, LTR-6-2 and LTR-6-3 are for 6 h after the initiation of treatment; LTR-12-1, LTR-12-2 and LTR-12-3 are for 12 h after the initiation of treatment. Three replicates for each time point. 9 samples in all.

2014-11-15 | E-GEOD-62315 | biostudies-arrayexpress

Grape Bud Dormancy -Vitis vinifera

Project description:Bud endodormancy induction response of two genotypes (Seyval, a hybrid white wine grape and Vitis riparia, PI588259, a native North American grape species) was compared under long (15 h) and short (13 h) photoperiods. Proteins were extracted from both genotypes for all time points and experimental conditions. The proteins were separaed by 2D-PAGE, trypsin digested, and the peptides identified with a MALDI-TOF-TOF mass spectrometer. A master gel was made and mapped with all proteins from both genotypes. The proteins were identified by matching the peptide sequences against the 8X Vitis vinifera grape genome in NCBI. This study was funded by NSF grant DBI064755 and is the result of a collaboration between Dr. Anne Fennell at South Dakota State University and Dr. Grant R. Cramer at the University of Nevada, Reno.

2010-08-09 | PRD000169 | Pride

High-throughput sequencing of small RNAs in Vitis vinifera

Project description:Vitis vinifera endogenous small RNAs Size fractionated small RNA from total RNA extracts of Vitis vinifera leaves, inflorescences, tendrils and small berries were ligated to adapters, purified again and reverse transcribed. After PCR amplification the sample was subjected to Solexa/Illumina high throughput pyrosequencing. Please see www.illumina.com for details of the sequencing technology.

2010-04-29 | E-GEOD-18405 | biostudies-arrayexpress

Vitis vinifera Transcriptome or Gene expression

Project description:Seedless varieties are of particular importance to the table-grape and raisin industries. Gibberellin (GA) application is widely used in the early stages of seedless berry development to increase berry size and economic value. However, the underlying mechanism of GA induction of berry enlargement is not well understood. Here, RNA-sequencing analysis of âCentennial Seedlessâ (Vitis vinifera L.) berries treated with GA3 12 days after flowering is reported.

2014-12-05 | E-MTAB-4247 | biostudies-arrayexpress

Esca proper and apoplexy on trunk wood of Vitis vinifera L. cv.Chardonnay

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2014-06-25 | PXD000015 | Pride

High-throughput sequencing of small RNAs in Vitis vinifera

Project description:Vitis vinifera endogenous small RNAs

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RNA-Seq data of Vitis vinifera cv. Furmint berry samples (Tokaj wine region) exposed to noble rot

Project description:White grape (Vitis vinifera cv. Furmint) berry samples subjected to natural noble rot were collected in a vineyard in Mád, Hungary (Tokaj wine region). Raw data include grapevine and Botrytis cinerea sequence reads.

2021-11-25 | E-MTAB-11205 | biostudies-arrayexpress

Novel functional microRNAs from virus-free and infected Vitis vinifera plants under water stress.

Project description:Size fractionated small RNA from total RNA extracts of Vitis vinifera leaves were ligated to adapters, purified again and reverse transcribed. After PCR amplification the sample was subjected to Illumina high throughput pyrosequencing. The kit used is TrueSeq Small RNA kit Please see www.illumina.com for details of the sequencing technology.

2016-02-02 | GSE63244 | GEO

microRNA-target RNA pairs revealed by Parallel analysis of RNA ends in Vitis vinifera

Project description:Vitis vinifera RNA degradome Isolated polyadenylated RNA from total RNA extracts of Vitis vinifera leaves, were ligated to 5'-adapter that include san MmeI recognition site. The ligated products were purified again, reverse transcribed and cleaved with MmeI. The 5' fragments were purified from gel and to a 3'- dsDNA adapter and PCR amplified. After PCR amplification the sample was subjected to Solexa/Illumina high throughput pyrosequencing. Please see www.illumina.com for details of the sequencing technology.

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