Arabidopsis thaliana
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ABSTRACT:
OTHER RELATED OMICS DATASETS IN: E-GEOD-62537
PROVIDER: PRJNA264365 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:Identification of genes differentially expressed in roots of Arabidopsis Col-0 and ndr1-1 mutants 48 h post inoculation with the fungal pathogen Verticillium longisporum.
2014-11-20 | GSE62537 | GEO
Project description:Expression data roots of Arabidopsis plants inoculated with Verticillium longisporum
2016-05-03 | E-GEOD-62537 | ExpressionAtlas
Project description:Identification of genes differentially expressed in roots of Arabidopsis Col-0 and ndr1-1 mutants 48 h post inoculation with the fungal pathogen Verticillium longisporum. Arabidopsis Col-0 and ndr1-1 seeds were grown in petri dishes with liquid MS medium supplemented with 0.2% sucrose. After two weeks on a horizontal shaker (40 rpm), plants were rinsed twice in sterile distilled water and transferred to liquid 0.5xMS medium without sucrose, supplemented with 200 conidia ml-1 of V. longisporum isolate VL1 (CBS110220). Control plants were grown in 0.5x MS only. At 48 hours post inoculation, materials were harvested in biological replicates (>50 plant roots) and immediately frozen in liquid nitrogen. RNA was extracted with Aurum Total RNA Mini Kit (Bio-Rad) and hybridized to the Arabidopsis ATH1 Genome Array at the DNAVision facility (Gosselies, Belgium). Microarray assays were performed with triplicate biological root samples. Normalization of the microarray data was performed using the Robust Multichip Average (RMA) method as implemented by the “quantile normalization” function in the Bioconductor 1.3 software.
2014-11-20 | E-GEOD-62537 | biostudies-arrayexpress
Project description:Verticillium longisporum is a soil-borne fungal pathogen causing vascular disease predominantly in oilseed rape. The pathogen enters its host through the roots and entertains a parasitic life stage in the xylem before invading other tissues late in the infection cycle. We have started to approach the question how and when the host plant senses the colonization of the xylem using Arabidopsis thaliana as a model plant. Although the stress-related phytohormones salicylic acid, jasmonic acid and abscisic acid increase only at 28 to 35 days, expression of V. longisporum-induced genes (VliGs) starts in the leaf veins as early as 5 dpi when disease symptoms and fungal DNA cannot yet be detected. It is concluded that an elicitor is transported from the root to the aerial parts. More than one third of the VliGs identified by whole genome expression profiling at 18 dpi encode apoplastically localized proteins involved in cell wall modifications and potential defense responses. The identified VliGs provide a useful tool to elucidate the contribution of the induced genes to the disease phenotype and the defense response. Moreover, they will help to identify the elicitor(s) and the components of the signal transduction chain that shape the V. longisporum – plant interaction. Keywords: Arabidopsis, cell wall, microarray, phytohormones, Verticillium longisporum, xylem
2008-03-25 | GSE10385 | GEO
Project description:Resistance against Verticillium longisporum is quantitative and multigenic. The vec1 QTL in Arabidopsis thaliana controlled resistance against systemic colonization by the fungus. Gene expression was studied to identify genes and pathways controlled by vec1. Near-isogenic lines derived from the resistant ecotype Bur and the susceptible ecotype Ler were compared. NIL9 contained Ler-alleles in the genomic region between 11753 and 12285 kb within vec1 on chromosome 2, tmNIL130 contained Bur-alleles. Microarrays were performed on samples containing the hypocotyl and the shoot basis because colonization is stopped in this region in resistant genotypes. Two developmental stages were studied: time-point (1) at the onset of flowering, when systemic colonization was shown to start and time point (2) at the onset of silique maturity, when extensive fungal proliferation was shown to occur. Two pathogen treatments were studied: (1) mock-inoculated controls, (2) plants inoculated with the V. longipsorum isolate 43 (VL).
2015-06-30 | GSE70021 | GEO
Project description:Bacterial wilt caused by Ralstonia solanacearum is a serious seed/soil borne disease that causes severe yield and quality losses in many plants. In order to understand the change in genome expression of inculated plants, microarray analysis were performed. Twenty one days old roots of Arabidopsis Col-0 were inoculated with Ralstonia solanacearum race 4 @ 10^9 & 10^8 cfu/ml in different plants, distil water were mock inoculated, after five days plants were taken for RNA extraction and hybridization on Affymetrix microarrays. Plants were incubated in growth chamber for disease development, temperature and humidity were maintained as per plant requirement for both treated and control plants.
2016-12-21 | GSE92631 | GEO
Project description:Resistance against Verticillium longisporum is quantitative and multigenic. The vec1 QTL in Arabidopsis thaliana controlled resistance against systemic colonization by the fungus. Gene expression was studied to identify genes and pathways controlled by vec1. Near-isogenic lines derived from the resistant ecotype Bur and the susceptible ecotype Ler were compared. NIL9 contained Ler-alleles in the genomic region between 11753 and 12285 kb within vec1 on chromosome 2, tmNIL130 contained Bur-alleles. Microarrays were performed on samples containing the hypocotyl and the shoot basis because colonization is stopped in this region in resistant genotypes. Two developmental stages were studied: time-point (1) at the onset of flowering, when systemic colonization was shown to start and time point (2) at the onset of silique maturity, when extensive fungal proliferation was shown to occur. Two pathogen treatments were studied: (1) mock-inoculated controls, (2) plants inoculated with the V. longipsorum isolate 43 (VL). Gene expression was studied in two genotypes (NIL9-susceptible; tmNIL130-resistant), under two pathogen treatments (mock-inoculated; VL-inoculated) at two time points (at the onset of flowering, 13 days post inoculation [dpi]; at the onset of silique maturity, 27-29 dpi). Three biological replicates were used for each treatment (24 microarrays altogether).
2015-06-30 | E-GEOD-70021 | biostudies-arrayexpress
Project description:Plant roots secrete secondary metabolites to sense the enviroment around them. Among them, terpenes play a prominent role. Terpenes can have either fungistatic or fungicide action. However, their exact role in plant-host interactions is not fully understood. Verticillium longisporum is a soilborne pathogen causing disease in Brasicacae plants. In this project we investigated the transcriptomic changes of this species upon exposure to the β-pinene monoterpene in different time points 0hpi, 8hpi, 24hpi and 24hpi.
2021-11-07 | GSE158956 | GEO
Project description:Transcriptional profiling of Arabidopsis thaliana roots comparing inoculated roots with a bacterial strain Antarctic (Pseudomonas sp, Da-bac TI-8) vs untreated roots (control)
2013-03-28 | GSE45559 | GEO
Project description:The goal of this project is to compare the primary metabolite profile in different tissue types of the model plant Arabidopsis thaliana. Specifically, plants were grown hydroponically under the long-day (16hr light/day) condition at 21C. Tissue samples, including leaves, inflorescences, and roots were harvest 4 1/2 weeks post sowing. Untargeted primary metabolites profiling was carried out using GCTOF.
2021-12-09 | ST002012 | MetabolomicsWorkbench