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Ovis aries

ABSTRACT: Maternal nutrition induces gene expression changes in fetal muscle and adipose tissues in sheep

PROVIDER: PRJNA266236 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR1639090.fastq.gz	Fastqsanger.gz
	SRR1639100.fastq.gz	Fastqsanger.gz
	SRR1639110.fastq.gz	Fastqsanger.gz
	SRR1639091.fastq.gz	Fastqsanger.gz
	SRR1639101.fastq.gz	Fastqsanger.gz

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Transcriptome sequencing of Ovis aries

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Transcriptome sequencing of Ovis aries

Project description:We have completed the high quality reference genome for domestic sheep (Oar v3.1) and performed a detailed survey of gene expression across different tissues. RNA-seq data of 7 tissue types from the reference female Texel and skin tissue from a Gansu alpine fine wool sheep were sequenced. Here is the part of the RNA-seq data sequenced in BGI, including 7 tissue types from the reference female Texel and skin type from a Gansu alpine fine wool sheep.

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Maternal nutrition induces gene expression changes in fetal muscle and adipose tissues in sheep

Project description:Maternal nutrition during different stages of pregnancy can induce significant changes in the structure, physiology, and metabolism of the offspring. These changes could have important implications on food animal production especially if these perturbations impact muscle and adipose tissue development. The objective of this study was to evaluate the effect of different maternal diets on the transcriptome of fetal tissues in sheep. Ewes were bred to a single sire and from days 67 ± 3 of gestation until necropsy (days 130 ± 1), they were fed one of three isoenergetic diets: alfalfa haylage (H; fiber), corn (C; starch), or dried corn distillers grains (D; fiber plus protein plus fat). Longissimus dorsi (M), subcutaneous adipose depot (S), and perirenal adipose depot (R) tissues from individual fetuses were pooled and then analyzed by RNA sequencing. A total of 26 fetuses were removed from 15 dams. From the fetuses three different tissues were collected including one muscle, longissimus dorsi muscle (M), and two adipose tissues, perirenal adipose depot (R) and subcutaneous adipose depot (S). The RNA samples from the 26 fetuses were pooled to generate four biological replicates per maternal diet and tissue. In particular, per each diet and tissue, two RNA pools were created from male fetuses and two RNA pools were created from female fetuses. Overall, a total of 36 pools (i.e., 12 pools per tissue, 3 tissues in total) underwent RNA extraction, library generation, and subsequent sequencing.

2014-11-04 | E-GEOD-62938 | biostudies-arrayexpress

Maternal nutrition induces gene expression changes in fetal muscle and adipose tissues in sheep

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MeDIP sequencing of Ovis aries

Project description:We have completed the high quality reference genome for domestic sheep (Oar v3.1).

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MeDIP sequencing of Ovis aries

Project description:We have completed the high quality reference genome for domestic sheep (Oar v3.1). Early-stage Illumina GA sequence platform sequenced less reads in high GC content regions than in other regions. To read through higher GC content regions, we generated 2 Gb MeDIP-seq data for filling gaps in sheep reference genome assembly.

2014-06-06 | E-GEOD-56644 | biostudies-arrayexpress

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Project description:Expression data from fetal sheep immunocytes

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