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Arabidopsis thaliana

ABSTRACT: Genome-wide analysis of wild type and gemin2 mutant plants [cold exposure]

OTHER RELATED OMICS DATASETS IN: E-GEOD-63406E-GEOD-63405

PROVIDER: PRJNA267681 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR1655110_1.fastq.gz	Fastqsanger.gz
	SRR1655110_2.fastq.gz	Fastqsanger.gz
	SRR1655120_1.fastq.gz	Fastqsanger.gz
	SRR1655120_2.fastq.gz	Fastqsanger.gz
	SRR1655111_1.fastq.gz	Fastqsanger.gz

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Cold induced changes: differences between the Arabidopsis thaliana wild type and freezing sensitive mutants.

Project description:Arabidopsis sfr mutants are deficient in cold acclimation during exposure to coolnon-freezing temperatures. Although not visibly affected by the cold they have lost the ability to survive subsequent freezing. We plan to investigate how the sfr2 and sfr6 mutants respond to low temperature on the gene expression level. Wild type plants that have undergone identical treatments in parallel are necessary controls. The cold treatment of plants in the rosette stage (soil grown in a 8/16 hours day/night cycle) will be carried out in a cooled growth chamber at 4 degrees for 24 hours (same light regimetreatment starting/ending at the 4th hour of light). The aerial parts of the treated and untreated plants will be collected and frozen immediately in liquid nitrogen for RNA extraction. Comparison of the cold response of thousands of Arabidopsis genes in the wild type to the situation in our freezing sensitive mutants will enhance our understanding of the cold response itself and illuminate the effect of the mutations on the cold acclimation process. Experimenter name = Irene Bramke Experimenter phone = 01784 44 3770 Experimenter fax = 01784 43 4326 Experimenter address = Royal Holloway Experimenter address = University of London Experimenter address = School of Biological Sciences Experimenter address = Bourne Building Experimenter address = Laboratory 406 Experimenter zip/postal_code = TW20 OEX Experimenter country = UK Keywords: growth_condition_design; genetic_modification_design

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Project description:Genome-wide analysis of wild type and gemin2 mutant plants exposed to cold

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