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Penicillium oxalicum

ABSTRACT: Transcriptomic analysis of fungus Penicillium oxalicum and its laeA and creA deletion strains

PROVIDER: PRJNA267684 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR1655164.fastq.gz	Fastqsanger.gz
	SRR1655165.fastq.gz	Fastqsanger.gz
	SRR1655166.fastq.gz	Fastqsanger.gz
	SRR1655167.fastq.gz	Fastqsanger.gz

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Similar Datasets

Transcriptomic analysis of fungus Penicillium oxalicum and its laeA and creA deletion strains

Project description:The goals of this study are to compare differential gene expressions for Penicillium oxalicum wild type strain (WT), laeA knockout strain (M-NM-^TlaeA), creA knockout strain (M-NM-^TcreA), and double genes knockout strain (M-NM-^TlaeAM-NM-^TcreA). The deletion of laeA downregulated genes involved in oxidation- reduction process, alkaloid metabolic process, and transmembrane transport. We find the expression levels of seven secondary metabolism gene clusters (totally 28 clusters) were silenced inM-NM-^TlaeA. The deletion of creA upregulated genes involved in hydrolase activity, acting on glycosyl bonds. Many genes involved in conidiation were drastically regulated inM-NM-^TlaeAM-NM-^TcreA. This study provides the information that combined laeA and creA function are required in conidiation and hydrolase activity of P. oxalicum. Examination of differential gene expressions by digital gene expression tag profiling in Penicillium oxalicum wild type strain and three mutant strains (laeA knockout strain, creA knockout strain and double genes knockout strain). qRTM-bM-^@M-^SPCR validation was performed using SYBR Green assays.

2014-11-19 | E-GEOD-63408 | biostudies-arrayexpress

Transcriptomic analysis of fungus Penicillium oxalicum and its laeA and creA deletion strains

Project description:The goals of this study are to compare differential gene expressions for Penicillium oxalicum wild type strain (WT), laeA knockout strain (ΔlaeA), creA knockout strain (ΔcreA), and double genes knockout strain (ΔlaeAΔcreA). The deletion of laeA downregulated genes involved in oxidation- reduction process, alkaloid metabolic process, and transmembrane transport. We find the expression levels of seven secondary metabolism gene clusters (totally 28 clusters) were silenced inΔlaeA. The deletion of creA upregulated genes involved in hydrolase activity, acting on glycosyl bonds. Many genes involved in conidiation were drastically regulated inΔlaeAΔcreA. This study provides the information that combined laeA and creA function are required in conidiation and hydrolase activity of P. oxalicum.

2014-11-19 | GSE63408 | GEO

Transcriptomic analysis of fungus Penicillium oxalicum and its laeA deletion strains in 24h and 60h

Project description:The goals of this study are to compare differential gene expressions for Penicillium oxalicum wild type strain (WT), and laeA knockout strain (ΔlaeA) in different development phase. The deletion of laeA downregulated genes involved in oxidation- reduction process, alkaloid metabolic process, and transmembrane transport. We find the expression levels of seven secondary metabolism gene clusters (totally 28 clusters) were silenced inΔlaeA. This study provides the information that laeA function are required in conidiation and hydrolase activity of P. oxalicum. Examination of differential gene expressions by digital gene expression tag profiling in Penicillium oxalicum wild type strain and laeA knockout mutant strains in 24h and 60h in modified Czapek culture medium with 2% glucose as carbon resource. qRT–PCR validation was performed using SYBR Green assays.

2015-07-27 | E-GEOD-71287 | biostudies-arrayexpress

Penicillium oxalicum

Project description:Transcriptomic analysis of fungus Penicillium oxalicum and its laeA deletion strains in 24h and 60h

| PRJNA291069 | ENA

Transcriptomic analysis of fungus Penicillium oxalicum and its laeA deletion strains in 24h and 60h

2015-07-27 | GSE71287 | GEO

Transcriptomic analysis of cellulolytic fungus Penicillium oxalicum and transcription factor mutant strains in response to different carbon sources

2015-05-28 | GSE69298 | GEO

Transcriptomic analysis of fungus Penicillium oxalicum and its laeA, clrB and rcoA deletion strains

Project description:The goals of this study are to compare differential gene expressions for Penicillium oxalicum wild type strain (WT), laeA knockout strain (ΔlaeA), rcoA knockout strain (ΔrcoA), and clrB knockout strain (ΔclrB). The deletion of laeA downregulated genes involved in oxidation- reduction process, alkaloid metabolic process, and transmembrane transport. We find the expression levels of seven secondary metabolism gene clusters (totally 28 clusters) were silenced inΔlaeA. The deletion of clrB downregulated genes involved in hydrolase activity, acting on glycosyl bonds. Many genes involved in conidiation were drastically regulated in ΔrcoA. This study provides the information that combined laeA, clrB and rcoA function are required in conidiation and hydrolase activity of P. oxalicum.

2021-03-22 | GSE112203 | GEO

Transcriptomic analysis of cellulolytic fungus Penicillium oxalicum and transcription factor mutant strains in response to different carbon sources

Project description:RNA-seq was used to compare the responses of Penicillium oxalicum strains to different carbon sources including carbon-starvation, glucose and cellulose. The wild-type strain and transcription facor (ClrB, CreA and AmyR) mutants were studied. A total of 24 samples were analzyed, which include biological triplicates. The wild-type strain cultured without carbon source was considered as a reference.

2015-05-28 | E-GEOD-69298 | biostudies-arrayexpress

Penicillium oxalicum

Project description:Transcriptomic analysis of cellulolytic fungus Penicillium oxalicum and its sporogenesis related genes deletion strains

| PRJNA294261 | ENA

Transcriptomic analysis of cellulolytic fungus Penicillium oxalicum and its sporogenesis related genes deletion strains

Project description:RNA-seq was used to compare differential gene expressions for Penicillium oxalicum wild type strain (M12) and sporognesis related genes knock out strains.The goals of this study are to construct the sporogenesis regulation pathway of Penicillium oxalicum. Examination of differential gene expressions by digital gene expression tag profiling in Penicillium oxalicum wild type strain and nine mutant strains (flbA knoutout strain, flbB knoutout strain, flbC knoutout strain, flbD knoutout strain, flbE knoutout strain,wetA knoutout strain, abaA knoutout strain,stuA knoutout strain, swi6 knoutout strain)

2015-09-01 | E-GEOD-72523 | biostudies-arrayexpress

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