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Drosophila melanogaster

ABSTRACT: AGO1 immunoprecipitated small RNAs from D. melanogaster wildtype and mutant ovaries

PROVIDER: PRJNA268781 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR1664731.fastq.gz	Fastqsanger.gz
	SRR1664732.fastq.gz	Fastqsanger.gz
	SRR1664734.fastq.gz	Fastqsanger.gz
	SRR1664736.fastq.gz	Fastqsanger.gz

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Impact of nuclear Piwi elimination on chromatin state in Drosophila melanogaster ovaries

Project description:The piRNA-interacting Piwi protein is involved in transcriptional silencing of transposable elements in ovaries of D. melanogaster. Here we characterized the genome-wide effect of nuclear Piwi elimination on the presence of the heterochromatic H3K9me3 mark and HP1a, as well as on the transcription-associated mark H3K4me2. Our results demonstrate that a significant increase in the H3K4me2 level upon nuclear Piwi loss is not accompanied by the alterations in H3K9me3 and HP1a levels for several germline-expressed transposons, suggesting that in this case Piwi prevents transcription by a mechanism distinct from H3K9 methylation. We found that the targets of Piwi-dependent chromatin repression are mainly related to the elements that display a higher level of H3K4me2 modification in the absence of silencing, i.e. most actively transcribed elements. We also show that Piwi-guided silencing does not significantly influence the chromatin state of dual-strand piRNA-producing clusters. In addition, host protein-coding gene expression is essentially not affected due to the nuclear Piwi elimination, but we noted an increase in small nuclear spliceosomal RNAs abundance and propose Piwi involvement in their posttranscriptional regulation. Our work reveals new aspects of transposon silencing in Drosophila, indicating that transcription of transposons can underpin their Piwi dependent silencing, while canonical heterochromatin marks are not obligatory for their repression. Examination of histone modifications in ovaries from two different fly lines- piwiNt/piwi2 (mutant) and piwi/+ (wildtype)

2014-05-19 | E-GEOD-56347 | biostudies-arrayexpress

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