Project description:We focused on how mica fine particle influences macrophage activities. We analyzed altered transcription in RAW 264.7 cells at 0, 12, and 48 h following the stimulation with 100 M-BM-5g/mL of mica fine particles.
Project description:Global transcriptomic profiles of blood monocytes from Hmox1 homozygous knockout mice compared to wild type controls following Mtb infection
Project description:We report the application of RNA sequencing to determine the transcriptional response of primary bone marrow macorphages and murine macrophage Raw 264.7 cells in response to stimulation with LPS/IFNgamma(M1) or IL-4 (M2) stimulation conditions
Project description:The purpose of this study was to characterize global gene expression in human airway epithelial cells and identify cellular pathways associated with coarse, fine and ultrafine particulate matter (PM) exposures. Ambient PM was collected in 3 different size fractions from Chapel Hill air, particles were extracted from foam or filter matrices and lyophilized. Human primary airway epithelial cells were exposed to particles at 250μg/ml or vehicle control for 6h in culture. Following exposure, RNA was isolated and hybridized to human HG U133A affymetrix chips. Keywords: particle treatment
