Mus musculus
Ontology highlight
ABSTRACT:
PROVIDER: PRJNA269179 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:Dynamic changes in chromatin states during specification and differentiation of adult intestinal stem cells [k27ac]
| PRJNA352945 | ENA
Project description:Dynamic changes in chromatin states during specification and differentiation of adult intestinal stem cells [mbd]
| PRJNA352952 | ENA
Project description:Dynamic changes in chromatin states during specification and differentiation of adult intestinal stem cells [h2az]
| PRJNA352932 | ENA
Project description:Dynamic changes in chromatin states during specification and differentiation of adult intestinal stem cells [k4m3]
| PRJNA352933 | ENA
Project description:Dynamic changes in chromatin states during specification and differentiation of adult intestinal stem cells [k27m3]
| PRJNA352948 | ENA
Project description:Dynamic changes in chromatin states during specification and differentiation of adult intestinal stem cells [rnaseq]
| PRJNA352951 | ENA
Project description:Metabolism is vital to cellular function and tissue homeostasis during human lung development. In utero, embryonic pluripotent stem cells undergo endodermal differentiation towards a lung progenitor cell fate that can be mimicked in vitro using induced human pluripotent stem cells (hiPSCs) to study genetic mutations. To identify differences between wild type and surfactant protein B (SFTPB)-deficient cell lines during endoderm specification towards lung, we used an untargeted metabolomics approach to evaluate the developmental changes in metabolites. We found that the metabolites most enriched during the differentiation from pluripotent stem cell to lung progenitor cell, regardless of cell line, were sphingomyelins and phosphatidylcholines, two important lipid classes in fetal lung development. The SFTPB mutation had no metabolic impact on early endodermal lung development. The identified metabolite signatures during lung progenitor cell differentiation may be utilized as biomarkers for normal embryonic lung development.
2021-06-29 | ST002055 | MetabolomicsWorkbench
Project description:Expression data during vascular endothelial cells differentiation from mouse embryonic stem cells
| PRJNA309159 | ENA
Project description:Expression data from embryonic stem cells differentiation
| PRJNA253990 | ENA
Project description:During mammalian pre-implantation development, the cells of the blastocyst’s inner cell mass differentiate into the epiblast and primitive endoderm lineages, which give rise to the fetus and extra-embryonic tissues, respectively. Extra-embryonic endoderm differentiation can be modeled in vitro by induced expression of GATA transcription factors in mouse embryonic stem cells. Here we use this GATA-inducible system to quantitatively monitor the dynamics of global proteomic changes during the early stages of this differentiation event and also investigate the fully differentiated phenotype, as represented by embryo-derived extra-embryonic endoderm (XEN) cells. Using mass spectrometry-based quantitative proteomic profiling with multivariate data analysis tools, we reproducibly quantified 2,336 proteins across three biological replicates and have identified clusters of proteins characterized by distinct, dynamic temporal abundance profiles. We first used this approach to highlight novel marker candidates of the pluripotent state and extra-embryonic endoderm differentiation. Through functional annotation enrichment analysis, we have shown that the downregulation of chromatin-modifying enzymes, the re-organization of membrane trafficking machinery and the breakdown of cell-cell adhesion are successive steps of the extra-embryonic differentiation process. Thus, applying a range of sophisticated clustering approaches to a time-resolved proteomic dataset has allowed the elucidation of complex biological processes which characterize stem cell differentiation and could establish a general paradigm for the investigation of these processes.
2015-06-13 | PXD001901 | Pride