Project description:Genomic Run On (GRO): determination of the nascent transcriptional rates and mRNA levels in several yeast mutants (SAC3, SUS1, SRC1)
Project description:Determination of transcriptional rates and mRNA levels during osmotic stress in S. cerevisiae wild type and cbc1/sto1 mutant cells using Genomic Run On (GRO)
Project description:We analyzed the effect of SPT16 on the transcription rates, mRNA stabilities and mRNA levels by doing GRO experiments in a thermosensitive spt16 point mutant (G132D) mutant at 35 ºC comparing with permisive temperature (30 ºC) and a wt treated in the same way.
