Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Chromobacterium violaceum strain:GN5

ABSTRACT: Chromobacterium violaceum strain:GN5 Genome sequencing and assembly

PROVIDER: PRJNA269999 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	JWPW01.dat.gz	Other
	JWPW01.fasta.gz	Fasta.gz
	JWPW01.master.dat	Other
	JWPW01.dat.gz	Other

Items per page:

1 - 4 of 4

Similar Datasets

Identification of the OsbR regulon in Chromobacterium violaceum

Project description:Here, we described a novel transcriptional regulator belonging to the MarR family that we named OsbR (oxidative stress response and biofilm formation regulator) in the opportunistic pathogen Chromobacterium violaceum. Transcriptome profiling by DNA microarray using strains with deletion or overexpression of osbR showed that OsbR exert a global regulatory role in C. violaceum, regulating genes involved in oxidative stress response, nitrate reduction, biofilm formation, and several metabolic pathways. EMSA assays showed that OsbR binds to the promoter regions of several OsbR-regulated genes and the in vitro DNA binding activity was inhibited by oxidants. We demonstrated that the overexpression of osbR caused activation of ohrA even in the presence of the repressor OhrR, which resulted in improved growth under organic hydroperoxide treatment. We showed that the proper regulation of the nar genes by OsbR ensures an optimal growth of C. violaceum under anaerobic conditions by tuning the reduction of nitrate to nitrite. Finally, the osbR overexpressing strain showed reduction in biofilm formation and this phenotype correlated with the OsbR-mediated repression of two gene clusters encoding putative adhesins.

2021-04-11 | GSE171860 | GEO

Identification of the EmrR regulon in Chromobacterium violaceum

Project description:Antibiotic resistance can arise by several mechanisms, including mutation in transcription factors that regulate drug efflux pumps. In this work, we identified EmrR as a MarR family transcription factor involved in antibiotic resistance in Chromobacterium violaceum, a Gram-negative bacterium that occurs in soil and water and can act as a human opportunistic pathogen. Antibiogram and minimum inhibitory concentration (MIC) assays showed that the ΔemrR mutant presented increased resistance to the antibiotic nalidixic acid in respect to the wild-type strain. The emrR gene is near to a putative operon emrCAB, which encode the efflux pump EmrCAB. DNA Microarray analysis showed that EmrR represses the emrCAB operon and some other putative transporters. Northern blot assays validated that EmrR represses the emrCAB operon and this repression can be released by salicylate, but not other compounds such as nalidixic acid or ethidium bromide. Electrophoretic mobility shift assays (EMSA) showed that EmrR binds directly to the promoter regions of emrR, emrCAB and other genes to exert negative regulation. Therefore, in response to compounds as salicylate, EmrR derepresses the operon emrCAB causing overexpression of the efflux pump EmrCAB and increased resistance to nalidixic acid in C. violaceum.

2018-11-01 | GSE112521 | GEO

Chromobacterium violaceum

Project description:Chromobacterium violaceum Genome sequencing and assembly

| PRJNA699412 | ENA

Chromobacterium violaceum

Project description:Chromobacterium violaceum Genome sequencing

| PRJNA413993 | ENA

Chromobacterium violaceum strain:OUAT_2017

Project description:Chromobacterium violaceum strain:OUAT_2017 Genome sequencing and assembly

| PRJNA764546 | ENA

Chromobacterium violaceum strain:RDN09

Project description:Chromobacterium violaceum strain:RDN09 Genome sequencing and assembly

| PRJNA640761 | ENA

Chromobacterium violaceum strain:LK15

Project description:Chromobacterium violaceum strain:LK15 Genome sequencing and assembly

| PRJNA277407 | ENA

Chromobacterium violaceum strain:LK30

Project description:Chromobacterium violaceum strain:LK30 Genome sequencing and assembly

| PRJNA277426 | ENA

Chromobacterium violaceum strain:M23796

Project description:Chromobacterium violaceum strain:M23796 Genome sequencing and assembly

| PRJNA747180 | ENA

Chromobacterium violaceum strain:GHPS1

Project description:Chromobacterium violaceum strain:GHPS1 Genome sequencing and assembly

| PRJNA387362 | ENA

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data