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Multiple: Clostridium acetobutylicum, Clostridium cellulolyticum, Escherchia coli

ABSTRACT: Multiple: Clostridium acetobutylicum, Clostridium cellulolyticum, Escherchia coli Genome sequencing

PROVIDER: PRJNA270067 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR1707090_1.fastq.gz	Fastqsanger.gz
	SRR1707090_2.fastq.gz	Fastqsanger.gz
	SRR1707141_1.fastq.gz	Fastqsanger.gz
	SRR1707141_2.fastq.gz	Fastqsanger.gz
	SRR1707134_1.fastq.gz	Fastqsanger.gz

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Project description:Clostridium acetobutylicum is a Gram-positive, endospore-forming bacterium that is considered as a strict anaerobe. It ferments sugars to the organic acids acetate and butyrate or shifts to formation of the solvents - ethanol, butanol and acetone. In most bacteria the major regulator of iron homeostasis is Fur (ferric uptake regulator). Analysis of the genome of Clostridium acetobutylicum has revealed three genes encoding Fur-like proteins. The amino acid sequece of one of them showed 70% similarity to the Fur protein of the closely related Bacillus subtilis.<br>Thus, to gain insight into the role of Fur and the mechanisms for maintenance of iron homeostasis in this strict anaerobic organism, we determined its transcriptional profile in response to iron limitation and inactivation of fur.

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Project description:Clostridium acetobutylicum is a typical bacterium of major importance to industrial butanol production. In order to dissect the regulatory network pertaining to the industrial application of this bacterium, catabolite control protein A (CcpA) was investigated for its global function by DNA microarray.It showed that CcpA of C. acetobutylicum controls hundreds of genes, not only carbon metabolism, but also solvent production and sporulation in the life cycle.The results here demonstrated that CcpA is an important pleiotropic regulator related to some specific physiological and biochemical process in butanol-producing C. acetobutylicum.

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