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Arabidopsis thaliana

ABSTRACT: A SAM oligomerization domain shapes the genomic binding landscape of the LEAFY transcription factor

PROVIDER: PRJNA270526 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR1719111.fastq.gz	Fastqsanger.gz
	SRR1719112.fastq.gz	Fastqsanger.gz
	SRR1719113.fastq.gz	Fastqsanger.gz
	SRR1719114.fastq.gz	Fastqsanger.gz
	SRR1719115.fastq.gz	Fastqsanger.gz

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A SAM oligomerization domain shapes the genomic binding landscape of the LEAFY transcription factor

Project description:Multicellular organisms develop new structures by initiating specific gene regulatory programs through coordinated changes at the transcriptional and chromatin levels. Master transcription factors orchestrate such global changes but the underlying molecular mechanisms remain unclear. Here we focus on LEAFY (LFY), a key regulator of flower development in angiosperms. The resolution of the crystallographic structure of its N-terminal domain shows that it forms an unanticipated Sterile Alpha Motif (SAM) domain. This domain drives LFY oligomerization, which we establish to be essential for LFY floral switch function. Combining in vitro and in vivo experiments, we demonstrate that oligomerization facilitates LFY binding to DNA regions with multiple binding sites. Moreover, genome-wide analyses reveal an additional prominent role for the oligomerization domain: it confers upon LFY the capacity to bind to closed chromatin regions. This novel property, combined with its previously demonstrated capacity to recruit chromatin remodelers, indicates that LFY controls floral fate by acting as a plant pioneer transcription factor.

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Project description:A biophysical DNA binding model for the LEAFY transcription factor reveals the evolutionary fluidity of its binding sites

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Project description:We investigated the transcriptional effects of p63 binding by analyzing ME180 cells depleted for all p63 isoforms via expression of a small hairpin RNA (shRNA) targeting the p63 oligomerization domain. Keywords: differential expression profiling from transcription factor depletion

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Genome wide binding sites of the Arabidopsis B3 domain protein FUSCA3

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Project description:Transcription factor Interplay between LEAFY and APETALA1/ CAULIFLOWER during Floral Initiation

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Project description:Transcription factor Interplay between LEAFY and APETALA1/ CAULIFLOWER during Floral Initiation

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