Project description:Recent studies have revealed that microRNAs (miRNAs) participate in all steps of cancer initiation and progression by regulating protein coding genes at the post-transcriptional level. MiRNAs are in turn regulated by other genes, forming a complex regulatory network. The regulation networks between the p53 tumor suppressor and miRNAs in nasopharyngeal carcinoma (NPC) remain unclear. The aim of this study was to investigate the regulatory roles of the TP53 gene in regulating miRNA expression profiles in NPC cell line HNE2.
Project description:Recent studies have revealed that long non-coding RNAs (lncRNAs) participate in all steps of cancer initiation and progression by regulating protein coding genes at the epigenetic, transcriptional and post-transcriptional levels. LncRNAs are in turn regulated by other genes, forming a complex regulatory network. The regulation networks between the p53 tumor suppressor and lncRNAs in nasopharyngeal carcinoma (NPC) remain unclear. The aim of this study was to investigate the regulatory roles of the TP53 gene in regulating lncRNA and mRNA expression profiles in NPC cell line HNE2. p53 induced gene expression in human nasopharyngeal carcinoma cell line HNE2 was measured at 0, 12, 24 and 48 hours after transfected by pCMV-p53 plasmid.
Project description:microRNA profiles of exosomes :Exosomes from two nasopharyngeal carcinoma cell line TW03M-oM-<M-^HEBV+M-oM-<M-^Iand TW03M-oM-<M-^HEBV-M-oM-<M-^I and Exosomes from nasopharyngeal epithelial cells NP69 Two-condition experiment, Exosomes from two nasopharyngeal carcinoma cell line vs.one normal epithelium cell line. Biological replicates:1 Exosomes from nasopharyngeal carcinoma cell line TW03M-oM-<M-^HEBV+M-oM-<M-^I, 1 Exosomes from nasopharyngeal carcinoma cell line TW03M-oM-<M-^HEBV-M-oM-<M-^I,1 Exosomes from nasopharyngeal epithelial cells NP69.
Project description:This SuperSeries is composed of the following subset Series: GSE15047: Nasopharyngeal carcinoma cell lines (expression analysis) GSE15051: Nasopharyngeal carcinoma cell lines (copy number analysis) This study was designed to screen genes overexpression due to copy number gain.
Project description:microRNA profiles of exosomes :Exosomes from two nasopharyngeal carcinoma cell line TW03（EBV+）and TW03（EBV-） and Exosomes from nasopharyngeal epithelial cells NP69
Project description:The incidence of TP53 loss-of-function in hepatocellular carcinoma is very high. In order to clarify the gene expression differences induced by the changes of TP53 gene, we used two human hepatocellular carcinoma cell lines, SK-HEP-1 and Hep 3B with TP53 knockdown or overexpression for RNA sequencing . SK-HEP-1 is a TP53 wild-type hepatocellular carcinoma cell line. Thus, we knockdown TP53 in SK-HEP-1. Hep 3B is a TP53 loss-of-function hepatocellular carcinoma cell line. Thus, we overexpress TP53 in Hep 3B. Results of RNA-seq analysis showed the differences after knocking-down or overexpressing TP53.
