Genome activity during the emergence of mouse embryonic totipotency
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ABSTRACT:
PROVIDER: PRJNA271561 | ENA |
REPOSITORIES: ENA
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Project description:Fertilization transforms sperm and egg into a totipotent embryo but the underlying mechanisms are unknown. We here report that gene expression initiates during the gamete-to-embryo transition in mouse embryos. Meiotic exit induced by sperm entry enhances a transcriptionally-permissive epigenetic landscape. Time-course analysis of single embryos revealed a succession of genome-wide transcription 'ripples' initiating within 2 hours. Disrupting key pluripotency transcription factor levels prior to sperm entry had little immediate effect, indicating that different mechanisms engender pluripotent and totipotent states. These findings suggest that a hierarchical gene expression program characterizes the emergence of totipotency during the gamete-to-embryo transition, with broad mechanistic implications for the reprogramming of cellular potency.
2023-03-02 | GSE64648 | GEO
Project description:Fertilization transforms sperm and egg into a totipotent embryo but the underlying mechanisms are unknown. We here report that gene expression initiates during the gamete-to-embryo transition in mouse embryos. Meiotic exit induced by sperm entry enhances a transcriptionally-permissive epigenetic landscape. Time-course analysis of single embryos revealed a succession of genome-wide transcription 'ripples' initiating within 2 hours. Disrupting key pluripotency transcription factor levels prior to sperm entry had little immediate effect, indicating that different mechanisms engender pluripotent and totipotent states. These findings suggest that a hierarchical gene expression program characterizes the emergence of totipotency during the gamete-to-embryo transition, with broad mechanistic implications for the reprogramming of cellular potency.
2023-03-02 | GSE64649 | GEO
Project description:This SuperSeries is composed of the SubSeries listed below.
2023-03-02 | GSE64650 | GEO
Project description:Chromosomes are not randomly packed and positioned into the nucleus but folded in higher-order chromatin structures with defined functions. However, the genome of a fertilized embryo undergoes a dramatic epigenetic reprogramming characterized by extensive chromatin relaxation and the lack of a defined three-dimensional structure. This reprogramming is followed by a slow genome refolding that gradually strengthens the chromatin architecture during preimplantation development. Interestingly, genome refolding during early development coincides with a progressive loss of developmental potential suggesting a link between chromatin organization and cell plasticity. In agreement, loss of chromatin architecture upon depletion of the insulator transcription factor CTCF in embryonic stem cells led to the upregulation of the transcriptional program found in totipotent cells of the embryo, those with the highest developmental potential. This essay will discuss the impact of genome folding in controlling the expression of transcriptional programs involved in early development and their plastic-associated features.
| S-EPMC10281046 | biostudies-literature
Project description:Embryonic genome instability upon DNA replication timing program emergence
| PRJNA1075049 | ENA
Project description:Embryonic genome instability upon DNA replication timing program emergence
| PRJNA939700 | ENA
Project description:Genome activity during the emergence of mouse embryonic totipotency (morpholino)
| PRJNA271564 | ENA
Project description:Totipotency is the ability of a single cell to give rise to all the differentiated cells that build the conceptus, yet how to capture this property in vitro remains incompletely understood. Defining totipotency relies upon a variety of assays of variable stringency. Here we describe criteria to define totipotency. We illustrate how distinct criteria of increasing stringency can be used to judge totipotency by evaluating candidate totipotent cell types in the mouse, including early blastomeres and expanded or extended pluripotent stem cells. Our data challenge the notion that expanded or extended pluripotent states harbor increased totipotent potential relative to conventional embryonic stem cells under in vivo conditions.
2020-10-13 | GSE145609 | GEO
Project description:Zygotic genome activation (ZGA) marks the first transcriptional milestone and establishes embryonic totipotency. Although pioneer factors have been reported to initiate this process, how chromatin is primed for the totipotent state, allowing the binding of pioneer factors for successful ZGA, remains unclear. Here, we identify IntS11, the catalytic subunit of the Integrator complex, as a totipotent determinant of embryonic chromatin governing ZGA in Drosophila. We show that IntS11 functions upstream of pioneer factors in early embryos: maternal IntS11 depletion substantially impairs RNA polymerase II (Pol II) recruitment, thereby preventing pioneer factors Zelda and GAGA factor (GAF) from accessing regulatory elements and initiating genome-wide zygotic transcription. Mechanistically, IntS11 exerts dual roles: its canonical endonuclease activity is required to sustain major-wave zygotic transcription, while a distinct enzyme-independent function drives de novo Pol II loading and pioneer factor engagement. These findings uncover a fundamental maternal-specific mechanism whereby IntS11 establishes transcriptional competence, ensuring totipotent chromatin states and successful ZGA.
2026-03-31 | GSE299646 | GEO
Project description:Genome activity during the emergence of mouse embryonic totipotency (time course)
| PRJNA271563 | ENA