Project description:Microarray analysis on livers of young (three weeks old) Nile rats (Arvicanthis niloticus) fed with either a high carbohydrate diet only (percentage energy from carbohydrate:fat:protein = 70:10:20, 16.7 kJ/g) or a high carbohydrate diet supplemented with palm fruit juice (PFJ) (415 ml of 13000 ppm gallic acid equivalent (GAE) for a final concentration of 5.4 g GAE per kg diet or 2.7 g per 2000 kcal) PFJ (415 ml of 13000 ppm gallic acid equivalent (GAE) for a final concentration of 5.4 g GAE per kg diet or 2.7 g per 2000 kcal) was supplemented to young Nile rats (Arvicanthis niloticus) given a high carbohydrate diet (percentage energy from carbohydrate:fat:protein = 70:10:20, 16.7 kJ/g) to observe for possible anti-diabetic effects. Livers were harvested four weeks after the feeding regimen for gene expression studies. Results from the microarray data analysis carried out show that rats given PFJ had down-regulated insulin signalling, consistent with anti-diabetic effects observed in vivo. Total RNA obtained from livers of young Nile rats (Arvicanthis niloticus) given PFJ in a high carbohydrate diet (four weeks after the feeding regimen) were compared to controls (three replicates in the treatment group versus four replicates in the control group)
Project description:Microarray analysis on livers of young (three weeks old) Nile rats (Arvicanthis niloticus) fed with either a high carbohydrate diet only (percentage energy from carbohydrate:fat:protein = 70:10:20, 16.7 kJ/g) or a high carbohydrate diet supplemented with palm fruit juice (PFJ) (415 ml of 13000 ppm gallic acid equivalent (GAE) for a final concentration of 5.4 g GAE per kg diet or 2.7 g per 2000 kcal) PFJ (415 ml of 13000 ppm gallic acid equivalent (GAE) for a final concentration of 5.4 g GAE per kg diet or 2.7 g per 2000 kcal) was supplemented to young Nile rats (Arvicanthis niloticus) given a high carbohydrate diet (percentage energy from carbohydrate:fat:protein = 70:10:20, 16.7 kJ/g) to observe for possible anti-diabetic effects. Livers were harvested four weeks after the feeding regimen for gene expression studies. Results from the microarray data analysis carried out show that rats given PFJ had down-regulated insulin signalling, consistent with anti-diabetic effects observed in vivo.
Project description:To quantify gene expression differences in olfactory epithelium between the mouse (Mus musculus) and the Nile rat (Arvicanthis niloticus), paired-end RNA sequencing (RNA-seq) was used to profile olfactory epithelium transcriptomes of six Nile rats and six mice (C57BL/6J) (one male and one female at the age of 8, 12, and 16 weeks for each species).
Project description:Analysis of the effect of using a fruit and vegetable juice concentrate to reduce systemic inflammation in obesity. The hypothesis tested whether the presence of polyphenols in the fruit and vegetable juice concentrate could reduce the expression of systemic inflammatory genes in the blood of Obese patients with high levels of plasma CRP (≥3.0). Results provide evidence that systemic inflammatory genes/ and or pathways may be modulated by the fruit and vegetable juice concentrate.
