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Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

ABSTRACT: Transcriptional Analysis of Deinococcus radiodurans for Novel sRNAs expression that are Differentially Expressed under Ionizing Radiation

PROVIDER: PRJNA272579 | ENA |

REPOSITORIES: ENA

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Dataset's files

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			Action	DRS
	SRR1757502_1.fastq.gz	Fastqsanger.gz
	SRR1757502_2.fastq.gz	Fastqsanger.gz
	SRR1757503_1.fastq.gz	Fastqsanger.gz
	SRR1757503_2.fastq.gz	Fastqsanger.gz
	SRR1757504_1.fastq.gz	Fastqsanger.gz

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Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539 strain:R1 | cultivar:ATCC

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Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

Project description:Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539 Genome sequencing and assembly

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Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

Project description:Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539 Genome sequencing and assembly

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Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

Project description:Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539 Genome sequencing and assembly

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Deinococcus radiodurans R1: wildtype strains vs. oxyR disruption strains

Project description:In Deinococcus radiodurans, a previously unreported special characteristic of DrOxyR (DR0615) is found with only one conserved cysteine. dr0615 gene mutant is hypersensitive to H2O2, but a little to ionizing radiation. Site-directed mutagenesis and subsequent in vivo functional analyses revealed that the conserved cysteine (C210) is necessary for sensing H2O2, but its mutation did not alter the binding characteristics of OxyR on DNA. Under oxidant stress, DrOxyR is oxidized to sulfenic acid form, which can be reduced by reducing reagents. In addition, quantitative real-time PCR and global transcription profile results showed that OxyR is not only a transcriptional activator (e.g., katE, drb0125), but also a transcriptional repressor (e.g., dps, mntH). Transcriptional profiling of comparing wildtype strains with oxyR disruption strains under normal growth conditions. Keywords: Genetic modification

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Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539 strain:R1

Project description:Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539 strain:R1 Raw sequence reads

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Transcriptional Analysis of Deinococcus radiodurans for Novel sRNAs expression that are Differentially Expressed under Ionizing Radiation

Project description:In this study, we identified potential sRNA candidates in D. radiodurans by a whole transcriptome deep sequencing analysis, and confirm the expression of 31 sRNAs in D. radiodurans with Northern blotting analysis and RT-PCR. A total of 8 confirmed sRNAs showed differential expression during recovery after acute ionizing radiation (15 kGy).

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