Project description:Single nucleotide polymorphisms in intron 1 of the fat mass and obesity-associated (FTO) gene were found to be associated with an increased risk of adult obesity. Enhanced FTO expression in mice leads to hyperphagia, increased fat mass, and higher body weight. Neuronal-specific FTOâ??deleted mice have an identical lean body weight phenotype to global FTO-deleted mice. The physiological role of adipose FTO in the homeostasis of energy regulation remains to be elucidated. We used microarrays to elucidate the metabolic pathways that are regulated by FTO in the white fat. FTO flox/flox and Adiponectin-cre FTO flox/flox (AFO) mice were fed with chow diet. White fat tissues from epididymal adipose pad were harvested under ad lib condition for RNA isolation. Three independent pools of FTO flox/flox and AFO mouse white fat RNA were included in the study.
Project description:Analysis of visceral white adipose tissue (EWAT) from Yin Yang 1 adipose-specific knockout mice exposed to cold (4ºC) for 4 days. Control mice YY1flox/flox versus YY1flox/flox; Adiponectin Cre were cold exposed for 4 days.
Project description:Adipocyte-specific Atgl knockout (Atgladipo-/-) mice were bred at the animal facility of the University of Graz. They were originally generated at the animal facility of the University of Pittsburgh by mating the floxed-Atgl mouse strain with Adiponectin-Cre mice to obtain Cre+/−Atglflox/flox mice (AAKO). Cre-/-Atglflox/flox mice (Atgladipo+/+) were used as controls (CTRL).
Project description:We generated a Tcf7l2 F/F mouse and harvested preadipocytes rom these mice, immortalized them, and then transduced them with a retroviral vector containing CreERT2 and , differentiated them, performed ChIP-seq, and RNA-seq. We also generated and adipsoe specific knockout mouse where we crossed our Tcf7 F/Fmouse with an Adiponectin-Cre mice and performed RNA-seq on the inguimal white adipose tissue after 16 weeks of high fat diet, 65%
Project description:Purpose: The goals of this study are to investigate the mRNAs that bind to the FTO protein in the white fat tissue from mice. Methods: 1. White fat from CAG promoter driven transgenic Flag-tagged FTO mice were extracted with RNA protected. 2. Flag-FTO proteins were immunoprecipitated with control IP using IgG. 3. The immunoprecipitated RNAs were deep sequenced and analyzed. Results: We focused on the mRNAs which have functions related to lipid metabolism and homeostasis. Immunoprecipitated RNAs profiles from Flag-FTO IP and IgG IP were generated by deep sequencing.
Project description:We generated a Tcf7l2 F/F mouse and harvested preadipocytes from these mice, immortalized them, and then transduced them with a retroviral vector containing CreERT2 and , differentiated them, performed ChIP-seq, and RNA-seq. We also generated an adipose specific knockout mouse where we crossed our Tcf7 F/Fmouse with an Adiponectin-Cre mice and performed RNA-seq on the inguinal white adipose tissue after 16 weeks of high fat diet, 65% Conclusions: Adipsose selective deletion of Tcf7l2 led to hypertrophic inguinal white adipose tissue and impaired glucose metabolsim with transcripts involved in lipid and glucose metabolsim being altered.
Project description:Slc35d3 adipose-specific knockin (SAKI) mice were generated by crossbreeding mice carrying the Slc35d3 transgene locus controlled by loxp-stop-loxp sequence (LSL-Slc35d3) with adiponectin-derived Cre mice, which is a mouse model that expresses the cre enzyme specifically in adipose tissues. Male 6-week-old Slc35d3 adipose-specific knockin mice (SAKI group) and their control mice (wild-type mice, WT group) were fed a normal standard chow diet (NCD, 10 kal% fat, D09100304, Research Diets, Inc) for 10 weeks. All mice reached the experimental endpoint at 16 weeks of age. Mice in WT group were used as controls. Upon reaching the experimental endpoint, inguinal white adipose tissue (IngWAT) from three SAKI mice and three WT mice were removed for RNA sequencing.
Project description:We generated a Tcf7l2 F/F mouse and harvested preadipocytes rom these mice, immortalized them, and then transduced them with a retroviral vector containing CreERT2 and , differentiated them, performed ChIP-seq, and RNA-seq. We also generated and adipsoe specific knockout mouse where we crossed our Tcf7 F/Fmouse with an Adiponectin-Cre mice and performed RNA-seq on the inguimal white adipose tissue after 16 weeks of high fat diet, 65% Conclusions: Our study shows that knocking of Tcf7l2 results in enhanced adipocyte differentiation
