Mus musculus
Ontology highlight
ABSTRACT:
PROVIDER: PRJNA273728 | ENA |
REPOSITORIES: ENA
Dataset's files
| Action | DRS | |||
|---|---|---|---|---|
| SRR1774041.fastq.gz | Fastqsanger.gz | |||
| SRR1774042.fastq.gz | Fastqsanger.gz | |||
| SRR1774043.fastq.gz | Fastqsanger.gz | |||
| SRR1774044.fastq.gz | Fastqsanger.gz | |||
| SRR1774045.fastq.gz | Fastqsanger.gz |
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Project description:Purpose: To study the mechanisms involved in the regulation by NFIX on neural stem cell development and to examine the transcriptome changes associated with the loss of NFIX in neural stem cells. Methods: Subventricular zones of 10-day-old wild-type and Nfix KO mice were sectioned and dissociated into single cells. Cells were cultured in proliferation condition for 10 days. RNA was purified and poly-A selected to build the library for RNA-seq. Conclusions: Our study represents the first detailed analysis of transcriptome changes in primary monolayer-cultured neural stem cells associated with the loss of NFIX. Cells dissociated from 10-day-old wild-type and nuclear factor I-X (Nfix KO) mice subventricular zone were cultured in DMEM/F12 with B27, Glutamine, EGF and bFGF for 10 days. RNA was harvested with Norgen RNA purification micro kit and then prepared with illumina TruSeq kit. Samples from 6 mice (3 vs. 3) were loaded on one lane. 50-cycle single-read run was performed on Hiseq 2000. The sequence reads were analyzed by TopHat 2.0.7 followed by Cufflinks 1.3.0 with the mm9 UCSC annotation files.
2015-06-30 | E-GEOD-65337 | biostudies-arrayexpress
Project description:Purpose: To study the mechanisms involved in the regulation by NFIX on neural stem cell development and to examine the transcriptome changes associated with the loss of NFIX in neural stem cells. Methods: Subventricular zones of 10-day-old wild-type and Nfix KO mice were sectioned and dissociated into single cells. Cells were cultured in proliferation condition for 10 days. RNA was purified and poly-A selected to build the library for RNA-seq. Conclusions: Our study represents the first detailed analysis of transcriptome changes in primary monolayer-cultured neural stem cells associated with the loss of NFIX.
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2010-06-24 | E-GEOD-22476 | biostudies-arrayexpress
Project description:Neural stem cells from different brain regions show differencies in gene expression patterns and physiological functions. We used microarrays to find differential gene expression between the neuralstem cells from the subventricular zone of lateral ventricle and the subventricular zone of third ventricle. Cultured neural stem cells from embryonic day 17.5 mouse embryos were selected for RNA extraction and hybridization on Affymetrix microarrays. We sought to obtain embryonic neural stem cell cultures from three indipendent females.
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Project description:Expression data of miRNAs from adult neural progenitor cells in subventricular zone after stroke. Genome-wide profilings of miRNAs were measured in cultured non-ischemic (N=3) and ischemic SVZ (N=3) neural progenitor cells using miRNA microarray.
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Project description:Murine postnatal subventricular zone (SVZ) neural stem cells (NSCs): Wild-type (WT) vs. Dnmt3a-null (KO)
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