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Gadus morhua

ABSTRACT: Transcriptome analysis of Atlantic cod (Gadus morhua L.) from the brackish Baltic Sea

PROVIDER: PRJNA273805 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR1777740.fastq.gz	Fastqsanger.gz
	SRR1777742.fastq.gz	Fastqsanger.gz
	SRR1777743.fastq.gz	Fastqsanger.gz
	SRR1777734.fastq.gz	Fastqsanger.gz
	SRR1777745.fastq.gz	Fastqsanger.gz

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Microarray analysis of gene expression of Atlantic cod from different Baltic Sea regions

Project description:The Atlantic cod (Gadus morhua L.) is one of the most important species in the Baltic Sea with high ecological and economical value. To explore the differences in adaptation to salinity between Baltic cod from different regions, western (Kiel Bight) and eastern (Gdańsk Bay) samples were analyzed through oligonucleotide microarray.

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The study of Atlantic cod (Gadus morhua) spleen global gene expression response to intraperitoneal injection with formalin-killed atypical Aeromonas salmonicida.

Project description:This study was performed to validate the newly developed CGP Atlantic cod 20K oligonucleotide microarray. Atlantic cod (Gadus morhua) received an intraperitoneal injection of either formalin-killed, atypical Aeromonas salmonicida (Asal) or PBS and transcriptional profiles of spleen tissues from Asal-injected fish were compared to those from pre-injection control fish and PBS-injected control fish. Gene expression profiles resulting from this study were compared to those from suppression subtractive hybridization library studies, that were previously performed on the same samples, and to literature. Gene expression patterns of single genes were confirmed by QPCR analysis. This study has shown that the newly developed CGP Atlantic cod 20K oligo microarray platform is a valuable tool for cod genomic research.

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Effects on the lipid metabolism of Atlantic cod (Gadus morhua) after in vivo exposure with Ppar agonists

Project description:Lipid metabolism is essential in maintaining energy homeostasis in multicellular organisms. In vertebrates, the peroxisome proliferator-activated receptors (PPARs, NR1C) regulate the expression of many genes involved in these processes. Four Ppar subtypes from Atlantic cod (Gadus morhua) were recently cloned and characterized. However, the downstream regulatory role of Ppars in cod lipid metabolism is presently not well understood or described. Here we study the involvement of Atlantic cod Ppar subtypes in systemic regulation of lipid metabolism using the model agonists WY14,643, GW501516, and tetradecylthioacetic acid, employing a multiple omics approach after an in vivo exposure situation.

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