Streptococcus mutans
Ontology highlight
ABSTRACT:
PROVIDER: PRJNA274252 | ENA |
REPOSITORIES: ENA
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Project description:Nucleotide signaling pathways are found in all kingdoms of life and are utilized to coordinate a rapid response to changes in the environment. One more recently discovered signaling nucleotide is the secondary messenger cyclic diadenosine monophosphate (c-di-AMP), which is widely distributed among bacteria and is also found in several archaea. This cyclic nucleotide has been shown to involve in several important cellular processes, including maintenance of DNA integrity, cell wall metabolism, stress tolerance, transcription regulation and virulence. However, the mechanisms by which c-di-AMP modulates these physiological changes have remained largely unknown.In the present study, we identified and characterized a c-di-AMP synthase (CdaA) in S. mutans UA159. Furthermore, we investigated the role of CdaA in S. mutans cell physiology and global gene expression by utilizing cdaA gene in-frame deletion mutant. Our findings suggest that CdaA is an important global modulator of optimal growth and environmental adaption in this pathogen. Streptococcus mutans UA159 whole-genome arrays (8 x 15 K) were obtained from Agilent and included 1998 probes for S. mutans transcripts. For microarray analysis, S. mutans UA159 and S. mutans ?cdaA cells were routinely grown at 37°C anaerobically (90% N2, 5% CO2, 5% H2) in brain heart infusion broth (BHI; Difco, Sparks, MD, USA) to an optical density at 600 nm (OD600) of 0.5. Four RNA samples isolated from four independent cultures of UA159 and cdaA mutant strains were hybridized to the arrays and analyzed.
2015-04-06 | E-GEOD-65511 | biostudies-arrayexpress
Project description:Nucleotide signaling pathways are found in all kingdoms of life and are utilized to coordinate a rapid response to changes in the environment. One more recently discovered signaling nucleotide is the secondary messenger cyclic diadenosine monophosphate (c-di-AMP), which is widely distributed among bacteria and is also found in several archaea. This cyclic nucleotide has been shown to involve in several important cellular processes, including maintenance of DNA integrity, cell wall metabolism, stress tolerance, transcription regulation and virulence. However, the mechanisms by which c-di-AMP modulates these physiological changes have remained largely unknown.In the present study, we identified and characterized a c-di-AMP synthase (CdaA) in S. mutans UA159. Furthermore, we investigated the role of CdaA in S. mutans cell physiology and global gene expression by utilizing cdaA gene in-frame deletion mutant. Our findings suggest that CdaA is an important global modulator of optimal growth and environmental adaption in this pathogen.
2015-04-06 | GSE65511 | GEO
Project description:To investigate the inhibition mechanism of copper ions on S. mutans-V. parvula dual biofilm.
2023-02-01 | GSE222218 | GEO
Project description:Human pathogens use cell-cell communication systems, quorum sensing, to regulate virulence factors. Our recent work aiming at deciphering the quorum sensing regulon of Streptococcus mutans, a caries pathogen, revealed a locus encoding an atypical type II restriction-modification (R-M) system, DpnII, comprising two adenine methyltransferases. A recent survey of bacterial genomes demonstrated the widespread occurrence of methylated DNA, and the importance of DNA methylation was highlighted by the diverse processes in which they function. The present study aimed at deciphering the role of DNA methylation during S. mutans planktonic growth. A mutant strain, deficient in the DpnII R-M locus composed of the SMU.504 and SMU.505 genes encoding the two adenine methyltransferases and the SMU.506 gene encoding the DpnII restriction enzyme, was constructed in S. mutans wild-type (WT) strain. Transcriptome analysis was then performed to evaluate the differential planktonic gene expression between the WT cells and the DpnII R-M deficient cells.
2025-03-19 | GSE265802 | GEO
Project description:Transcriptional profiling of early logarithmic phase culture (O.D=0.2-0.3) of Streptococcus mutans UA159 comparing control of untreated Streptococcus mutans UA159 bacteria with Streptococcus mutans UA159 bacteria spplemented with 20µM synthetic DPD (pre-AI-2) which regulates gene expression via AI-2 quorum sensing system.Three compairisons were performed at pHs of 7,6 and 5.
2010-07-29 | GSE23159 | GEO
Project description:Transcriptional profiling to investigate the roles of ClpP and ClpX of S. mutans
2011-06-10 | GSE29871 | GEO
Project description:Transcriptional profiling to investigate the regulatory roles of SpxA and SpxB of S. mutans.
2009-12-25 | GSE19656 | GEO
Project description:RNA-seq was performed to examine the overall gene expression in a S. mutans ΔSMU.1147 strain and to identify potential genes that can mainly contribute to the changes of gene expression in S. mutans. Using RNA-seq technique, the differentially expressed genes that are implicated in phenotypic changes of the ΔSMU.1147 strain were studied.
2023-10-03 | GSE244268 | GEO
Project description:In the human pathogen Streptococcus mutans, the canonical peptide-based quorum sensing system is an inducible DNA repair system that is pivotal for bacterial survival. Previous work showed that the CSP signaling peptide is a stress-signaling alarmone that controls different stress-induced phenotypes. In this study, we exposed S. mutans to the CSP pheromone to mimic DNA damage conditions. Transcriptome analysis was then performed to evaluate the differential gene expression between the normal stationary phase cells and the CSP-induced stationary phase cells. The data obtained contribute to the understanding of the CSP-induced phenotypes in S. mutans.
2024-04-28 | GSE264670 | GEO