Project description:Cyanobacteria Prochlorococcus marinus subsp. pastoris str. CCMP1986 (MED4) and Prochlorococcus marinus str. MIT 9313 (MIT9313) are oceanic oxygenic phototrophs, where MED4 is abundant in surface waters (~0-50 meters) and MIT9313 is abundant at depths of ~100 meters. To explore nitrogen-regulated changes in gene expression in these Prochlorococcus ecotypes, log phase cultures of MED4 and MIT9313 were transferred to either nitrogen-replete (800 uM ammonium) or medium lacking supplemental nitrogen. Samples were taken over a time series in order to characterize changes in physiology and gene expression during increasing nitrogen starvation. The two ecotypes' molecular responses to different nitrogen sources were also assessed by comparing gene expression of log phase cultures growing in ammonium vs. urea and cyanate (MED4), and vs. urea and nitrite (MIT9313).

2007-10-11 | E-TABM-91 | biostudies-arrayexpress

Prochlorococcus marinus subsp. pastoris str. CCMP1986

Project description:Prochlorococcus marinus subsp. pastoris str. CCMP1986 Transcriptome or Gene expression

| PRJNA86895 | ENA

Prochlorococcus marinus subsp. pastoris str. CCMP1986

Project description:The transcriptome landscape of Prochlorococcus MED4 and the factors for stabilizing the core genome

| PRJNA214094 | ENA

Response of Prochlorococcus to varying CO2:O2 ratios

Project description:Carbon fixation plays a central role in determining cellular redox poise, increasingly understood to be a key parameter in cyanobacterial physiology. In the cyanobacterium Prochlorococcus--—the most abundant phototroph in the oligotrophic oceans--—the carbon-concentrating mechanism (CCM) is reduced to the bare essentials. Given the ability of Prochlorococcus populations to grow under a wide range of oxygen concentrations in the ocean, we wondered how carbon and oxygen physiology intersect in this minimal phototroph. We monitored genome-wide transcription in cells shocked with acute limitation of CO2, O2, or both. O2 limitation produced much smaller transcriptional changes than the broad suppression seen under CO2 limitation and CO2/O2 co-limitation. Strikingly, the transcriptional responses evoked by both CO2 limitation conditions were initially similar to that previously seen in high light stress, but at later timepoints we observed O2-dependent recovery of photosynthesis-related transcripts. These results suggest that oxygen plays a protective role in Prochlorococcus when carbon fixation is not a sufficient sink for light energy.

2015-04-15 | GSE65684 | GEO

Prochlorococcus marinus subsp. pastoris strain:CCMP1986

Project description:Prochlorococcus marinus subsp. pastoris strain:CCMP1986 Transcriptome or Gene expression

| PRJNA287445 | ENA