Musca domestica
Ontology highlight
ABSTRACT:
PROVIDER: PRJNA275312 | ENA |
REPOSITORIES: ENA
Dataset's files
| Action | DRS | |||
|---|---|---|---|---|
| SRR1802200.fastq.gz | Fastqsanger.gz | |||
| SRR1802201.fastq.gz | Fastqsanger.gz | |||
| SRR1802194.fastq.gz | Fastqsanger.gz | |||
| SRR1802195.fastq.gz | Fastqsanger.gz | |||
| SRR1802196.fastq.gz | Fastqsanger.gz |
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Project description:Hytrosaviridae family members replicate in the salivary glands (SGs) of their adult dipteran hosts and are transmitted to uninfected hosts via saliva during feeding. Despite inducing similar gross symptoms (SG hypertrophy; SGH), hytrosaviruses (SGHVs) have distinct pathobiologies, including sex-ratio distortions in tsetse flies and refusal of infected housefly females to copulate. Via unknown mechanism(s), SGHV replication in other tissues results in reduced fecundity in tsetse flies and total shutdown of vitellogenesis and sterility in housefly females. We hypothesized that vitellogenesis shutdown is caused by virus-induced modulation of hormonal titers. Here, we used RNA-Seq to investigate virus-induced modulation of host genes/pathways in healthy and virus-infected houseflies, and we validated expression of the most significantly modulated genes (n=23) by RT-qPCR. We also evaluated the levels and activities of hemolymph antimicrobial peptides (AMPs), levels of endogenous sesquiterpenoids, and impacts of exogenous hormones on ovarian development in viremic females. Of the 973 housefly unigenes that were significantly modulated (padj ≤ 0.01; log2FC ≤ or ≥ 2.0 ), 446 and 527 genes were downregulated and upregulated, respectively. While the most downregulated genes were related to reproduction (embryogenesis/oogenesis), the repertoire of upregulated genes was overrepresented by genes related to non-self recognition, ubiquitin-protease system, cytoskeletal traffic, cellular proliferation, development and movement, and snRNA processing. Overall, MdSGHV induced upregulation of components of the siRNA, innate antimicrobial immune, and autophagy pathways. MdSGHV reduced hemolymph sesquiterpenoids and completely shut down egg development in viremic females. However, the hormonal rescue of vitellogenesis did not result in egg production. The mechanism underlying MdSGHV-induced sterility has yet to be resolved.
2017-06-13 | GSE88939 | GEO
Project description:Impacts of the Hytrosavirus on the Transcriptome of the Housefly, Musca domestica
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Project description:Impacts of the Hytrosavirus on the Transcriptome of the Housefly, Musca domestica
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Project description:A Whole Transcriptional Linkage Analysis of Gene Co-Regulation in Insecticide Resistant House Flies, Musca domestica
| PRJNA170716 | ENA
Project description:Piperonyl butoxide (PBO) is an insecticide synergist known to inhibit the activity of cytochrome P450 enzymes. PBO is currently used in many insecticide formulations, and has also been suggested as a pre-treatment in some pesticide applications. Little is known about how insects respond to PBO exposure at the gene transcription level. We have characterised the transcriptional response of the Drosophila melanogaster genome after PBO treatment, using both a custom designed “detox” microarray containing cytochrome P450 (P450), glutathione S-transferase (GST) and esterase genes, and a full genome microarray. We identify a subset of P450 and GST genes, along with additional metabolic genes, that are highly induced by PBO. The gene set is an extremely similar gene set to that induced by phenobarbital, a compound for which pre-treatment is known to confer tolerance to a range of insecticide compounds. The implications of the induction of gene families known to metabolise insecticides and the use of PBO in pest management programs are discussed. Keywords: Induction response after treatment by PBO
2007-03-18 | GSE6144 | GEO
Project description:Illumina HiSeq 2000 RNASeq was conducted on the mRNA fraction of three adult female Musca domestica strains. Two strains were the insecticide susceptible strains CS and aabys, which has five distinct recessive morphological markers, one on each of the five autosomes in M. domestica. The third strain was ALHF, which is a highly insecticide resistant strain obtained in Alabama. The reference transcriptome was generated de novo using Trinity. The coding trimmed sequences from Augustus were then used as the mapping reference for the individual FASTQ files from each strain. trace reads were mapped using RSEM to generate FPKM estimates. Gene expression levels were compared to the ALHF strain using EdgeR to identify genes that were downregulated in both aabys and CS.
2014-01-02 | GSE39327 | GEO
Project description:Housefly (Musca domestica L.) associated microbiota changes continuously throughout the host life cycle
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Project description:Illumina HiSeq 2000 RNASeq was conducted on the mRNA fraction of three adult female Musca domestica strains. Two strains were the insecticide susceptible strains CS and aabys, which has five distinct recessive morphological markers, one on each of the five autosomes in M. domestica. The third strain was ALHF, which is a highly insecticide resistant strain obtained in Alabama. The reference transcriptome was generated de novo using Trinity. The coding trimmed sequences from Augustus were then used as the mapping reference for the individual FASTQ files from each strain. trace reads were mapped using RSEM to generate FPKM estimates. Gene expression levels were compared to the ALHF strain using EdgeR to identify genes that were downregulated in both aabys and CS. RNA Seq profiling of the mRNA of adult Musca domestica with different autosomal contributions from insecticide resistant and susceptible strains (whole insect pooled samples)
2014-01-02 | E-GEOD-39327 | biostudies-arrayexpress
Project description:The 791spin is the spinosad-selected strain derived from 791a, a laboratory strain derived from a multi-resistant field-collected sample of houseflies. The 791a strain proved highly resistant to pyrethroids and some anticholinesterases and showed some resistance to the chitin synthesis-disrupting larvicides.In order to understand the evolution of insecticide resistance, de novo assembly of a spinosad resistant housefly strain 791spin using 454 technology was initiated Transcriptome analysis of insecticide resistant housefly strain compared to susceptible strain
2015-05-12 | E-GEOD-65891 | biostudies-arrayexpress
Project description:Modifications of metabolic pathways are important in insecticide resistance evolution. Mutations leading to changes in expression levels or substrate specificities of cytochrome P450 (P450), glutathione-S-transferase (GST) and esterase genes have been linked to many cases of resistance with the responsible enzyme being shown to utilize the insecticide as a substrate. Many studies show that the substrates of enzymes are capable of inducing the expression of those enzymes. We investigated if this was the case for insecticides and the enzymes responsible for their metabolism. The induction responses for P450s, GSTs and esterases to six different insecticides were investigated using a custom designed microarray in Drosophila melanogaster. Even though these gene families can all contribute to insecticide resistance, their induction responses by insecticides is minimal. The insecticides spinosad, diazinon, nitenpyram, lufenuron and dicyclanil did not induce any P450, GST or esterase gene expression. DDT was the only insecticide tested capable of eliciting an induction response, but only low levels for one GST and one P450. These results are in contrast to the induction responses observed for the natural plant compound caffeine and the barbituate drug phenobarbital, both of which induced a number of P450 and GST genes to high. Our results show that although insects evolve metabolic resistance to insecticides, induction does not usually have a role in survival after insecticide application, and induction studies cannot be used to predict which genes are capable of metabolizing insecticides. This has implications for managing the evolution of metabolic insecticide resistance in natural insect populations. Keywords: induction response after treatment by various compounds
2007-03-18 | GSE5713 | GEO