Project description:Genomic assembly of nematode Wuchereria bancrofti, as part of the 50 Helminth Genomes Initiative sequencing of the parasitic worms that have the greatest impact on human, agricultural and veterinary disease and cause significant global health issues particularly in the developing world, or those used as model organisms.
Project description:Brugia pahangi is a parasitic nematode that is closely related to B. malayi and Wuchereria bancrofti. B. malayi and W. bancrofti are responsible for lymphatic filariasis, affecting around 120 million people in 73 countries worldwide.This project aims to undertake high-throughput sequencing of Brugia pahangi transcriptome. The objective is to use transcriptomics to support gene finding and to recognize genes expressed in given life stages.
Project description:The draft genome assembly of the Wolbachia endosymbiont of Wuchereria bancrofti (wWb) consists of 1060 850 bp in 100 contigs and contains 961 ORFs, with a single copy of the 5S rRNA, 16S rRNA and 23S rRNA and each of the 34 tRNA genes. Phylogenetic core genome analyses show wWb to cluster with other strains in supergroup D of the Wolbachia phylogeny, while being most closely related to the Wolbachia endosymbiont of Brugia malayi strain TRS (wBm). The wWb and wBm genomes share 779 orthologous clusters with wWb having 101 unclustered genes and wBm having 23 unclustered genes. The higher number of unclustered genes in the wWb genome likely reflects the fragmentation of the draft genome.