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TSA-Seq Mapping of Nuclear Genome Organization

ABSTRACT: TSA-Seq Mapping of Nuclear Genome Organization

PROVIDER: PRJNA275696 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR3538920.fastq.gz	Fastqsanger.gz
	SRR3538930.fastq.gz	Fastqsanger.gz
	SRR3538921.fastq.gz	Fastqsanger.gz
	SRR3538931.fastq.gz	Fastqsanger.gz
	SRR3538922.fastq.gz	Fastqsanger.gz

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Project description:We describe TSA-Seq, a new mapping method able to estimate mean chromosomal distances from nuclear speckles genome-wide and predict several Mbp chromosome trajectories between nuclear compartments without sophisticated computational modeling. Ensemble-averaged results reveal a clear nuclear lamina to speckle axis correlated with a striking spatial gradient in genome activity. This gradient represents a convolution of multiple, spatially separated nuclear domains, including two types of transcription ?hot-zones?. Transcription hot-zones protruding furthest into the nuclear interior and positioning deterministically very close to nuclear speckles have higher numbers of total genes, the most highly expressed genes, housekeeping genes, genes with low transcriptional pausing, and super-enhancers.

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