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Soil metagenome

ABSTRACT: The metagenomic analysis of soil from Leucaena leucocephala

PROVIDER: PRJNA276163 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR3085152.fastq.gz	Fastqsanger.gz
	SRR3085244.fastq.gz	Fastqsanger.gz
	SRR3087187.fastq.gz	Fastqsanger.gz
	SRR3087209_1.fastq.gz	Fastqsanger.gz
	SRR3087209_2.fastq.gz	Fastqsanger.gz

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Determination of genes expressed in response to drought in root and shoot of Leucaena leucocephala

2016-01-13 | GSE76810 | GEO

Determination of genes expressed in response to drought in root and shoot of Leucaena leucocephala

Project description:Leucaena leucocephala seedlings were treated with PEG6000 and the shoot and root tissues were collected after 48 hours following the treatment. The gene expressions were compared between treated and untreated in root and shoot separately. The differentially expressed genes may be related to drought resistance. RNA from shoot and root from treated and untreated L. leucocephala seedlings were extracted. Two biological replicates were made for each sample (each replicate represents about 10 individual seedlings).

2016-01-13 | E-GEOD-76810 | biostudies-arrayexpress

A comprehensive study to understand the effects of climate warming, simulated by soil transplant, on soil microbial community and its feedback responses

Project description:Soil transplant serves as a proxy to simulate climate change in realistic climate regimes. Here, we assessed the effects of climate warming and cooling on soil microbial communities, which are key drivers in Earth’s biogeochemical cycles, four years after soil transplant over large transects from northern (N site) to central (NC site) and southern China (NS site) and vice versa. Four years after soil transplant, soil nitrogen components, microbial biomass, community phylogenetic and functional structures were altered. Microbial functional diversity, measured by a metagenomic tool named GeoChip, and phylogenetic diversity are increased with temperature, while microbial biomass were similar or decreased. Nevertheless, the effects of climate change was overridden by maize cropping, underscoring the need to disentangle them in research. Mantel tests and canonical correspondence analysis (CCA) demonstrated that vegetation, climatic factors (e.g., temperature and precipitation), soil nitrogen components and CO2 efflux were significantly correlated to the microbial community composition. Further investigation unveiled strong correlations between carbon cycling genes and CO2 efflux in bare soil but not cropped soil, and between nitrogen cycling genes and nitrification, which provides mechanistic understanding of these microbe-mediated processes and empowers an interesting possibility of incorporating bacterial gene abundance in greenhouse gas emission modeling.

2013-10-30 | GSE51592 | GEO

Project description:Leucaena leucocephala mRNA Sequencing

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Project description:Leucaena leucocephala leaves and roots transcriptome

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A comprehensive study to understand the effects of climate warming, simulated by soil transplant, on soil microbial community and its feedback responses

Project description:Soil transplant serves as a proxy to simulate climate change in realistic climate regimes. Here, we assessed the effects of climate warming and cooling on soil microbial communities, which are key drivers in EarthM-bM-^@M-^Ys biogeochemical cycles, four years after soil transplant over large transects from northern (N site) to central (NC site) and southern China (NS site) and vice versa. Four years after soil transplant, soil nitrogen components, microbial biomass, community phylogenetic and functional structures were altered. Microbial functional diversity, measured by a metagenomic tool named GeoChip, and phylogenetic diversity are increased with temperature, while microbial biomass were similar or decreased. Nevertheless, the effects of climate change was overridden by maize cropping, underscoring the need to disentangle them in research. Mantel tests and canonical correspondence analysis (CCA) demonstrated that vegetation, climatic factors (e.g., temperature and precipitation), soil nitrogen components and CO2 efflux were significantly correlated to the microbial community composition. Further investigation unveiled strong correlations between carbon cycling genes and CO2 efflux in bare soil but not cropped soil, and between nitrogen cycling genes and nitrification, which provides mechanistic understanding of these microbe-mediated processes and empowers an interesting possibility of incorporating bacterial gene abundance in greenhouse gas emission modeling. Fifty four samples were collected from three soil types (Phaeozem,Cambisol,Acrisol) in three sites (Hailun, Fengqiu and Yingtan) along a latitude with reciprocal transplant; Both with and without maize cropping in each site; Three replicates in every treatments.

2013-10-30 | E-GEOD-51592 | biostudies-arrayexpress

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