Project description:To identify the expression of lncRNA in cow mammary glands infected with different pathogens, we used our custom lncRNA gene chip to explore expression of lncRNA from three Chinese cows. Compared with the control individual, a total of 629 and 139 lncRNAs showed significant different expression in mammary gland infected with S. aureus, and S. uberis, respectively. In addition, we found 3610, and 720 pairs of lincRNA/proteinencoding genes showing a highly significant correlated expression in mammary gland infected with S. aureus and S. uberis, respectively. Else, 309 and 98 pairs of lincRNA/proteinencoding genes showing a highly significant cis-regulation in mammary gland infected with S. aureus and S. uberis, respectively. Some of the lincRNAs expressed in mammary gland are located within quantitative trait loci for mastitis resistance. Our study first provides a glimpse into the lncRNA content of bovine mammary gland and will facilitate future experimental studies to unravel the function of these molecules. It may prove useful to elucidate their effects on mechanisms underlying the genetic variability of mastitis resistance.
Project description:Expression profiles of microRNAs from lactating and non-lactating bovine mammary glands and identification of miRNA related to lactation.
