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MicroRNA-mediated regulation of gene expression in the response of rice plants to fungal elicitors

ABSTRACT: MicroRNA-mediated regulation of gene expression in the response of rice plants to fungal elicitors

PROVIDER: PRJNA277438 | ENA |

REPOSITORIES: ENA

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Expression data from rice leaves treated or not with fungal elicitors and high-throughput pyrosequencing of endogenous small RNAs from Oryza sativa

Project description:High-throughput sequencing of small RNAs from rice was used to identify distinct miRNAs that are responsive to elicitors from the fungal pathogen Magnaporthe oryzae. [Expression profiling by array] We used microarrays to determine the expression behaviour of target genes for elicitor-regulated miRNAs. [High throughput sequencing] High-throughput sequencing of rice small RNAs was performed in two different tissues, leaves and roots, and two different time point of elicitor treatment, 30' and 2h Amplicons were prepared by 5M-BM-4and 3M-BM-4adaptor ligation in which the 5'-adaptor contained a 'barcode' consisting of a 4-nucleotide identifier sequence for each sample. The libraries containing unique barcodes were combined and subjected to pyrosequencing (454 Life SciencesTM, Roche) [Expression profiling by array] Leaves from rice plants were harvested at two time points after the onset of treatment (30' and 2h) with elicitors of Magnaporthe oryzae 18.1 and used for RNA extraction and hybridization on Affymetrix microarrays. Mock inoculations were performed with sterile water for control experiments. Three biological replicates were analyzed. Each sample represented a pool of approximately 150 rice plants. [High throughput sequencing] 8 samples examined: leaves and roots, treated or not with elicitors at two different time points, 30' and 2h (2x2x2)

2014-01-15 | E-GEOD-30583 | biostudies-arrayexpress

Study of bacterial and fungal elicitors on rice plant

Project description:Study of bacterial and fungal elicitors on rice plant

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Twig age-dependent negative correlation between Taxol accumulation and endophytic fungal assembles provide insights into screening efficient Taxol-related fungal elicitors

Project description:We identified two effective fungal elicitors, L01 (Guignardia) and J02 (Diaporthe). The two fungal elicitors might activate Taxol biosynthesis by regulating the MYC2a-controlled JA signaling pathway.

2024-04-08 | GSE263216 | GEO

Expression data from rice leaves treated or not with fungal elicitors and high-throughput pyrosequencing of endogenous small RNAs from Oryza sativa

2014-01-15 | GSE30583 | GEO

MicroRNA-mediated regulation of gene expression in the response of rice plants to fungal elicitors [degradome]

Project description:Elicitor-responsive miRNAs and their targets in rice

2016-03-06 | GSE66610 | GEO

MicroRNA-mediated regulation of gene expression in the response of rice plants to fungal elicitors [miRNA-Seq]

Project description:Elicitor-responsive miRNAs and their targets in rice

2016-03-06 | GSE66609 | GEO

MicroRNA-mediated regulation of gene expression in the response of rice plants to fungal elicitors.

Project description:MicroRNAs (miRNAs) are small non-coding RNAs that have important regulatory functions in plant growth, development, and response to abiotic stress. Increasing evidence also supports that plant miRNAs contribute to immune responses to pathogens. Here, we used deep sequencing of small RNA libraries for global identification of rice miRNAs that are regulated by fungal elicitors. We also describe 9 previously uncharacterized miRNAs in rice. Combined small RNA and degradome analyses revealed regulatory networks enriched in elicitor-regulated miRNAs supported by the identification of their corresponding target genes. Specifically, we identified an important number of miRNA/target gene pairs involved in small RNA pathways, including miRNA, heterochromatic and trans-acting siRNA pathways. We present evidence for miRNA/target gene pairs implicated in hormone signaling and cross-talk among hormone pathways having great potential in regulating rice immunity. Furthermore, we describe miRNA-mediated regulation of Conserved-Peptide upstream Open Reading Frame (CPuORF)-containing genes in rice, which suggests the existence of a novel regulatory network that integrates miRNA and CPuORF functions in plants. The knowledge gained in this study will help in understanding the underlying regulatory mechanisms of miRNAs in rice immunity and develop appropriate strategies for rice protection.

| S-EPMC4615225 | biostudies-literature

Small RNA profiling of Arabidopsis in response to fungal elicitors

Project description:This experiment describes small RNA gene expression after treatment of Arabidopsis seedlings with elicitors obtained from the fungus F. oxysporum

2014-12-01 | GSE59978 | GEO

MicroRNA-mediated regulation of gene expression in the response of rice plants to fungal elicitors

Project description:This SuperSeries is composed of the SubSeries listed below.

2016-03-06 | GSE66611 | GEO

Rice plants infested with planthoppers

Project description:Infestation with white-backed planthopper (WBPH) to rice caused induced resistance to rice pathogens but brown planthopper (BPH) infestation induce weaker resistance to rice pathogens. We compared changes in gene expression in rice plants infested with WBPH and BPH to gain some insight into the WBPH-induced resistance to rice pathogens. An analysis, using microarrays, of gene expression in rice plants infested with these planthoppers revealed that WBPH infestation caused high induction of many defense-related genes including pathogenesis-related (PR) genes than BPH infestation. Furthermore, hydroperoxide lyase 2 (OsHPL2) which is an enzyme to produce C6 volatiles was induced by WBPH infestation, but not by BPH infestation. Keywords: response to herbivory; induced resistance

2007-08-21 | GSE8811 | GEO

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