Project description:Developmental trajectories of gene expression reveal candidates for diapause termination, a key life history transition in the apple maggot fly, Rhagoletis pomonella

| PRJNA144307 | ENA

Rhagoletis pomonella isolate:Grant_MI

Project description:Rhagoletis pomonella isolate:Grant_MI Genome sequencing and assembly

| PRJNA328976 | ENA

Genome-wide variation and transcriptional changes in diverse developmental processes underly the rapid evolution of seasonality

Project description:Here, we present the results of an integrative study that leverages naturally segregating variation and a recent, adaptive divergence event affecting seasonal timing to identify developmental mechanisms underlying diapause progression in a tephritid fly, Rhagoletis pomonella. Also called the apple maggot fly, R. pomonella is native to North America, where it infests fruits of native Crataegus (hawthorn) species throughout its range. Derived populations of the fly infest apples (Malus domesticus), and thus have evolved in the last ~250 years since apples were introduced. Many molecular studies in conjunction with mark-recapture experiments document that the populations, or host races, remain genetically distinct despite ongoing gene flow, making R. pomonella a textbook example of incipient speciation with gene flow and host associated divergence. Strong natural selection on two primary traits, host finding behavior and seasonal timing, maintain genetic divergence. Both populations (hereafter apple and haw flies) have one generation per year, with a functionally obligate pupal diapause, overwintering in the soil. Adults must emerge coincident with host fruit availability, typically a period of only a few weeks, in order to successfully oviposit into fruits. Apple flies have evolved an earlier (~3 week) emergence timing to synchronize with apples, which fruit about 3 weeks earlier than hawthorn at a typical, sympatric site in the Midwest (e.g., Michigan or Illinois, USA). We combined RNA sequencing (RNAseq), phenotyping of emergence timing and brain morphology, and whole genome pooled resequencing (Poolseq) to infer mechanisms underlying segregating variation for diapause phenology in each Rhagoletic pomonella host race, and the evolved difference in phenology between host races.

2020-08-19 | GSE140473 | GEO

Identification of differentially methylated regions between leaves and young fruits of the apple GDDH13

Project description:The main objective of this analysis was to sequence the epigenome of the Apple (Malus domestica) doubled haploid 'Golden Delicious' tree. Our secondary objective was to identify differentially methylated regions between DNA purified from leaves and young fruits.

2016-09-17 | GSE87014 | GEO

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