Mus musculus
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ABSTRACT:
PROVIDER: PRJNA279591 | ENA |
REPOSITORIES: ENA
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Project description:Trichinella spiralis is a highly destructive parasitic nematode that invades and destroys intestinal epithelial cells, injures many different tissues during its migratory phase, and occupies and transforms myotubes during the final phase of its life cycle. Mice deficient in the IL-1 family receptor for the DAMP, IL-33 (called ST2), display reduced intestinal Th2 responses and impaired mast cell activation. IL-33 was constitutively expressed in intestinal epithelial cells, where it became concentrated in nuclei within 2 days of infection. Nuclear localization was an innate response to infection that occurred in intestinal regions where worms were actively migrating. We isolated intestinal epithelial cells from uninfected mice (cytoplasmic IL-33) and mice at 2 days post-infection (nuclear IL-33) to compare global expression profiles. We used microarrays to characterize the global gene expression that occurs in intestinal epithelial cells following T. spiralis-induced nuclear translocation of IL-33.
2015-03-28 | GSE67365 | GEO
Project description:Trichinella spiralis is a highly destructive parasitic nematode that invades and destroys intestinal epithelial cells, injures many different tissues during its migratory phase, and occupies and transforms myotubes during the final phase of its life cycle. Mice deficient in the IL-1 family receptor for the DAMP, IL-33 (called ST2), display reduced intestinal Th2 responses and impaired mast cell activation. IL-33 was constitutively expressed in intestinal epithelial cells, where it became concentrated in nuclei within 2 days of infection. Nuclear localization was an innate response to infection that occurred in intestinal regions where worms were actively migrating. We isolated intestinal epithelial cells from uninfected mice (cytoplasmic IL-33) and mice at 2 days post-infection (nuclear IL-33) to compare global expression profiles. We used microarrays to characterize the global gene expression that occurs in intestinal epithelial cells following T. spiralis-induced nuclear translocation of IL-33. Intestinal epithelial cells were isolated from Rag2-/- mice at day zero (uninfected) or two days post-infection with T. ispiralis for RNA extraction and hybridization on Affymetrix microarrays.
2015-03-28 | E-GEOD-67365 | biostudies-arrayexpress
Project description:Trichinellosis of human and other mammals was caused through the ingestion of the parasite，Trichinella spiralis，contaminated meat. It is a typical zoonotic disease that affects more than 10 million people world-wide. Parasites of Trichinella genus are unique intracellular pathogens. Adult Trichinella parasites directly release newborn larvae which invade striated muscle cells and causes diseases. In this study, we profiled the global transcriptome in the three developmental stages of T. spiralis. The transcriptomic analysis revealed the global gene expression patterns from newborn larval stage through muscle larval stage to adults. Thousands of genes with stage-specific transcriptional patterns were described and novel genes involving host-parasite interaction were identified. More than 45% of the protein-coding genes showed evidence of transcription from both sense and antisense strands which suggests the importance of RNA-mediated gene regulation in the parasite. This study presents a first deep analysis of the transcriptome of T. spiralis, providing insight information of the parasite biology.
2013-06-18 | GSE39151 | GEO
Project description:PURPOSE: To provide a detailed gene expression profile of the normal postnatal mouse cornea. METHODS: Serial analysis of gene expression (SAGE) was performed on postnatal day (PN)9 and adult mouse (6 week) total corneas. The expression of selected genes was analyzed by in situ hybridization. RESULTS: A total of 64,272 PN9 and 62,206 adult tags were sequenced. Mouse corneal transcriptomes are composed of at least 19,544 and 18,509 unique mRNAs, respectively. One third of the unique tags were expressed at both stages, whereas a third was identified exclusively in PN9 or adult corneas. Three hundred thirty-four PN9 and 339 adult tags were enriched more than fivefold over other published nonocular libraries. Abundant transcripts were associated with metabolic functions, redox activities, and barrier integrity. Three members of the Ly-6/uPAR family whose functions are unknown in the cornea constitute more than 1% of the total mRNA. Aquaporin 5, epithelial membrane protein and glutathione-S-transferase (GST) omega-1, and GST alpha-4 mRNAs were preferentially expressed in distinct corneal epithelial layers, providing new markers for stratification. More than 200 tags were differentially expressed, of which 25 mediate transcription. CONCLUSIONS: In addition to providing a detailed profile of expressed genes in the PN9 and mature mouse cornea, the present SAGE data demonstrate dynamic changes in gene expression after eye opening and provide new probes for exploring corneal epithelial cell stratification, development, and function and for exploring the intricate relationship between programmed and environmentally induced gene expression in the cornea. Keywords: other
2004-01-02 | GSE887 | GEO
Project description:Expression data from Control, Uninfected and BRAF infected cells
2025-01-24 | E-GEOD-46801 | ExpressionAtlas
Project description:Gene expression microarray of oral epithelial cells infected with Candida albicans
2025-01-23 | E-MTAB-7681 | ExpressionAtlas
Project description:We describe the first comprehensive study confirming the existence of DNA methylation, characterising the methylomes of three life stages of the food-borne agent of human trichinellosis, Trichinella spiralis. We further identify sets of genes where the DNA methylation status varied between thedevelopmental stages that are closely related to the parasitism of the organism. Examination of DNA methylation status in three life stages (Adult, muscle larve, new born larve) of Trchinella Spiralis using MethylC-seq.
2012-09-28 | E-GEOD-39328 | biostudies-arrayexpress
Project description:Trichinellosis of human and other mammals was caused through the ingestion of the parasiteM-oM-<M-^LTrichinella spiralisM-oM-<M-^Lcontaminated meat. It is a typical zoonotic disease that affects more than 10 million people world-wide. Parasites of Trichinella genus are unique intracellular pathogens. Adult Trichinella parasites directly release newborn larvae which invade striated muscle cells and causes diseases. In this study, we profiled the global transcriptome in the three developmental stages of T. spiralis. The transcriptomic analysis revealed the global gene expression patterns from newborn larval stage through muscle larval stage to adults. Thousands of genes with stage-specific transcriptional patterns were described and novel genes involving host-parasite interaction were identified. More than 45% of the protein-coding genes showed evidence of transcription from both sense and antisense strands which suggests the importance of RNA-mediated gene regulation in the parasite. This study presents a first deep analysis of the transcriptome of T. spiralis, providing insight information of the parasite biology. Messenger RNA from three developmental stages of T. spiralis was selectively purified from total RNA using oligo-(dT) conjugated magnetic beads. Complementary DNA (cDNA) was synthesized guided by oligo-(dT) as a primer.
2013-06-18 | E-GEOD-39151 | biostudies-arrayexpress
Project description:Transcription profiling by array of human A549 epithelial cells infected with poliovirus
2025-01-22 | E-MEXP-1072 | ExpressionAtlas