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Borreliella burgdorferi strain:B31-5A4

ABSTRACT: Borreliella burgdorferi strain:B31-5A4 Transcriptome or Gene expression

PROVIDER: PRJNA279595 | ENA |

REPOSITORIES: ENA

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Dataset's files

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			Action	DRS
	SRR1945080.fastq.gz	Fastqsanger.gz
	SRR1945081.fastq.gz	Fastqsanger.gz
	SRR1945082.fastq.gz	Fastqsanger.gz
	SRR1945083.fastq.gz	Fastqsanger.gz
	SRR1945084.fastq.gz	Fastqsanger.gz

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Project description:Transcriptional profiling of gene expression between parental strain B31 and rrp1 mutant. Cyclic-di-GMP is a bacterial second messenger that modulates many biological processes. Although its role in bacterial pathogenesis during mammalian infection has been widely recognized, the role of c-di-GMP in pathogen's life cycle in vector hosts is less understood. The enzootic cycle of the Lyme disease pathogen Borrelia burgdorferi involves both a mammalian host and an Ixodes tick vector. The B. burgdorferi genome encodes a single copy of the diguanylate cyclase gene (rrp1), which is responsible for the production of c-di-GMP. To determine the role of c-di-GMP in the life cycle of B. burgdorferi, an Rrp1-deficient B. burgdorferi strain was generated. The rrp1 mutant remains infectious in the mammalian host, but could not survive in the tick vector. To identify the mechanisms of Rrp1 contributing to B. burgdorferi pathogenesis and gene regulation, microarray was employed to compare gene expression profiles between the parental strain B31 and the rrp1 mutant.

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