Mus musculus
Ontology highlight
ABSTRACT:
PROVIDER: PRJNA280259 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:Expression data from embryonic stem cells differentiation
2015-08-04 | E-GEOD-58960 | ExpressionAtlas
Project description:Elf5 is a transcription factor with pivotal roles in the trophoblast compartment where it reinforces a trophoblast stem cell (TSC)-specific transcriptional circuit. However, Elf5 is also present in differentiating trophoblast cells that have ceased to express other TSC genes such as Cdx2 and Eomes. In the current study we aimed to elucidate the context-dependent role of Elf5 at the interface between TSC self-renewal and onset of differentiation. We demonstrate that precise levels of Elf5 are critical for normal expansion of the TSC compartment and embryonic survival, as Elf5 overexpression triggers precocious trophoblast differentiation. Through integration of protein interactome, transcriptome and genome-wide chromatin immunoprecipitation data we reveal that this abundance-dependent function is mediated through a shift in preferred Elf5 binding partners; in TSCs, Elf5 interaction with Eomes recruits Tfap2c to triply occupied sites at TSC-specific genes driving their expression. By contrast, the Elf5 and Tfap2c interaction becomes predominant as their protein levels increase. This triggers binding to double and single occupancy sites that harbour the cognate Tfap2c motif, causing activation of the associated differentiation-promoting genes. These data place Elf5 at the centre of a stoichiometry-sensitive transcriptional network where it acts as molecular switch governing the balance between TSC proliferation and differentiation.
2015-12-01 | PXD003145 | Pride
Project description:Brassica rapa Bra023154, AT1G31010 (E=1e-077) OSB4 | OSB4 (organellar single-stranded DNA binding protein 4); single-stranded DNA binding , is differentially expressed in 1 experiment(s);
| Bra023154 | ExpressionAtlas
Project description:Transcription profiling of mouse embryonic stem cell line Sox1Tv2 during neuronal differentiation
2015-11-02 | E-TABM-669 | ExpressionAtlas
Project description:This dataset is part of a study that aims to compare in vivo human trophoblast differentiation into EVTs to different in vitro trophoblast organoids using single-cell and single-nuclei RNA sequencing. This specific dataset includes scRNA-seq and snRNA-seq data from trophoblast stem cells (TSCs). Trophoblast stem cell (TSC) lines BTS5 and BTS11 derived by Okae and colleagues were grown as described previously (Okae et al. 2018) together with EVT differentiation media. This study shows that the main regulatory programs mediating EVT invasion in vivo are preserved in in vitro models of EVT differentiation from primary trophoblast organoids and trophoblast stem cells. Data for primary trophoblast organoids is available under E-MTAB-12650.
| EGAD00001010017 | EGA
Project description:Transcription profiling of murine J1 embryonic stem cells undergoing a differentiation time course to study changes in transcription during stem cell differentiation
2014-08-05 | E-GEOD-3749 | ExpressionAtlas
Project description:Brassica rapa Bra014089, AT1G47720 (E=5e-056) OSB1 | OSB1 (Organellar Single-stranded); single-stranded DNA binding , is expressed in 1 baseline experiment(s);
| Bra014089 | ExpressionAtlas
Project description:Z-DNA Binding Protein 1 Promotes Osteogenic Differentiation of Mesenchymal Stem Cells
| PRJNA481771 | ENA
Project description:Medicago truncatula MTR_1g083740, single-stranded DNA-binding protein, is differentially expressed in 2 experiment(s);
| MTR_1g083740 | ExpressionAtlas
Project description:Medicago truncatula MTR_1g070840, single-stranded DNA-binding protein, is differentially expressed in 2 experiment(s);
| MTR_1g070840 | ExpressionAtlas