Project description:To test the effect of chronic water avoidance stress on spinal gene expression profile in male wistar rats. Lumbar spinal cord samples were freshly isolated from rats exposed to daily water avoidance stress for 10 consecutive days. Samples were collected 24 hours after the last stressors.
Project description:To test the effect of chronic water avoidance stress on spinal microRNA expression profile in male wistar rats. Lumbar spinal cord samples were freshly isolated from rats exposed to daily water avoidance stress for 10 consecutive days. Samples were collected 24 hours after the last stressors.
Project description:Male infertility has become a global problem. And psychological stress has been proven to be an important cause of male infertility. In this work, we constructed a terrified-sound stress model in SD rats and performed single-cell RNA sequencing of the testicular tissues from the post-stressed and normal rats. Through the comparative analysis of the gene expression patterns of the 11,744 rat testicular cells, we revealed the potential mechanism by which terrified-sound induced psychological stress affects spermatogenesis and ultimately leads to reproductive impairment in male rats. This will provide a theoretical basis for the study of psychological stress on male reproductive impairments for other animals and humans, and further find the therapeutic targets for male infertility caused by psychological stress.
Project description:The study models of SCI were established in male Wistar rats with T10 spinal cord contusion. The rat bladders were collected fronm control group (CON group), 1 day after T10 operation group (1_D group), and 7 days after T10 operation group (7_D group), respectively. Then, samples were subjected to proteomic analysis using liquid chromatography-tandem mass spectrometry (LC-MS/MS) and intensive bioinformatic analysis were performed.
Project description:In the present study, we sought to understand the impact of obesity/metabolic disease (high-fat induced) on spinal cord injury (SCI) by examining transcriptome. Adult, male Long Evans rats received either thoracic level contusion of the spinal cord or sham laminectomy and then were allowed to recover on normal rat chow for 4 weeks and further on HFD for an additional 8 weeks. Spinal cord tissues harvested from the rats were processed for Affymetrix microarray and further transcriptomic analysis.
